• Species Reactivity

  Human

• Specificity

  Detects human DC-STAMP in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse DC-STAMP is observed.

• Source

  Monoclonal Mouse IgG    _2B Clone # 788524

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human DC-STAMP    
  Asp314-Thr376    
  Accession # Q9H295

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  2.5 µg/10    ⁶ cells

  See below

• 
  

• CyTOF-ready

  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DC-STAMP

DC-STAMP, also known as TM7SF4, is an approximately 50 kDa glycoprotein with seven transmembrane segments. It is expressed on the surface of dendritic cells and monocytes as well as on osteoclasts and their progenitors. DC-STAMP binds CTGF/CCN2 and cooperates with TRANCE/RANK L for inducing osteoclast differentiation and fusion into multinucleated cells. It contains a cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM) and associates with FC gamma RIII/CD16. Within aa 314-376, human DC-STAMP shares approximately 75% aa sequence identity with mouse and rat DC-STAMP.

• Long Name:

  Dendritic Cell-specific Transmembrane Protein

• Entrez Gene IDs:

  81501 (Human); 75766 (Mouse); 299889 (Rat)

• Alternate Names:

  DCSTAMP; DC-STAMP; dendritic cell-specific transmembrane protein; FIND; IL-4-induced Protein; TM7SF4; transmembrane 7 superfamily member 4