详细说明
Species Reactivity
Human
Specificity
Detects human Perilipin-2 in direct ELISAs.
Source
Monoclonal Mouse IgG 2B Clone # 788620
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Perilipin-2
Met1-His437
Accession # Q99541Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated
Applications
Recommended
ConcentrationSample
Western Blot
0.2 µg/mL
See below
Simple Western
4 µg/mL
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Western Blot | Detection of Human Perilipin-2 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and JAR human choriocarcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Perilipin-2 Monoclonal Antibody (Catalog # MAB7634) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # ). A specific band was detected for Perilipin-2 at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using . |
Simple Western | Detection of Human Perilipin-2 by Simple WesternTM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Perilipin-2 at approximately 58 kDa (as indicated) using 4 µg/mL of Mouse Anti-Human Perilipin-2 Monoclonal Antibody (Catalog # MAB7634). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody. |
Preparation and Storage
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Perilipin-2
Perilipin-2 (PLIN2), also known as Adipophilin (ADFP) and Adipose Differentiation-Related Protein (ADRP), is a widely expressed 52 kDa acylated protein that associates with membranes and cytoplasmic lipid droplets. It is upregulated during adipocyte differentiation and is a major component of milk fat globules. Perilipin-2 promotes the cellular uptake and accumulation of long chain fatty acids and regulates the intracellular distribution of phospholipids. It promotes hepatic insulin resistance in response to a high fat diet. Human Perilipin-2 shares 82% and 79% aa sequence identity with mouse and rat Perilipin-2, respectively.
Entrez Gene IDs:
123 (Human); 11520 (Mouse); 298199 (Rat)
Alternate Names:
ADFP; Adipophilin; Adipose differentiation-related proteinadipophilin; ADRP; MGC10598; perilipin 2; Perilipin2; Perilipin-2; PLIN2
粤公网安备44196802000105号
400-6226-992