• Species Reactivity

  Mouse

• Specificity

  Detects mouse PGLYRP2/PGRP-L in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse (rm) PGRP-S and rmPGRP-1 beta is observed.

• Source

  Monoclonal Rat IgG2a Clone # 439728

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse PGLYRP2/PGRP-L    
  Ser23-Asn530    
  Accession # Q8VCS0

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 750

Applications

• Recommended    
  Concentration

  Sample

• Intracellular Staining by Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  Mouse liver single‑cell suspension fixed with paraformaldehyde and permeabilized with saponin

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: PGLYRP2/PGRP-L

PGRP-L (peptidoglycan recognition protein‑long; also called PGRP-2 or TagL) is a 530 amino acid (aa) member of the PGRP family that is expressed in the liver. All mouse PGRPs bind peptidoglycan and Gram-positive bacteria, and may function as innate immunity pattern recognition molecules. PGRP-L is a Zn²⁺-dependent N‑acetylmuramoyl-L-alanine amidase that hydrolyzes cell wall glycopeptides. It contains two membrane-spanning segments, with both termini thought to be extracellular, but is also reported in serum. A truncated 450 aa isoform has been reported. Overall aa identity between mouse and human PGRP-L is 68%.

• Long Name:

  Peptidoglycan Recognition Protein Long/Peptidoglycan Recognition Protein 2

• Entrez Gene IDs:

  114770 (Human); 57757 (Mouse)

• Alternate Names:

  EC 3.5.1.28; peptidoglycan recognition protein 2tagl-beta; peptidoglycan recognition protein L; PGLYRP2; PGLYRPL; PGLYRPLHMFT0141; PGRP2; PGRPL; PGRP-L; PGRP-LN-acetylmuramoyl-L-alanine amidase; PGRPLPeptidoglycan recognition protein long; TAGL; tagL-alpha; TAGL-like