• Species Reactivity

  Human

• Specificity

  Detects human Neuromedin B R/NMBR. Stains human Neuromedin B R/NMBR transfectants but not irrelevant transfectants.

• Source

  Monoclonal Mouse IgG2a Clone # 466505

• Immunogen

  NS0 mouse myeloma cell line transfected with human NMBR    
  Met1-Met390    
  Accession # AAB27330

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 750

Applications

• Recommended    
  Concentration

  Sample

• Intracellular Staining by Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  HT‑29 human colon adenocarcinoma cell line fixed with paraformaldehyde and permeabilized with saponin

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Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Neuromedin B R/NMBR

Neuromedin B Receptor (NMBR) is an 80 kDa, 390 amino acid (aa) G-protein coupled 7-transmembrane glycoprotein receptor for bombesin-like peptides, binding Neuromedin B with high affinity and GRP (gastrin releasing peptide) with lower affinity.  Neuromedin B R expression in the olfactory and central thalamic regions of the brain plays a role in thermoregulation. It has also been shown to be mitogenic in colonic epithelium. Expression in the pituitary gland is important for regulation of the pituitary-thyroid axis. Within extracellular domains, human Neuromedin B R shares 86% and 82% aa identity with mouse and rat Neuromedin B R, respectively.

• Long Name:

  Neuromedin B Receptor

• Entrez Gene IDs:

  4829 (Human); 18101 (Mouse); 25264 (Rat)

• Alternate Names:

  Neuromedin B R; neuromedin B receptor; Neuromedin BR; neuromedin-B receptor; Neuromedin-B-preferring bombesin receptor; NMBR; NMB-R