• Species Reactivity

  Human

• Specificity

  Detects human ADRA1B. Stains human ADRA1B transfectants but not irrelevant transfectants.

• Source

  Monoclonal Mouse IgG2b Clone # 471802

• Immunogen

  NS0 mouse myeloma cell line transfected with human ADRA1B    
  Met1-Phe520    
  Accession # P35368

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 750

Applications

• Recommended    
  Concentration

  Sample

• Intracellular Staining by Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  PC‑3 human prostate cancer cell line fixed with paraformaldehyde and permeabilized with saponin

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Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: alpha-1B Adrenergic R/ADRA1B

ADRA1B, also known as the alpha -1B-adrenergic receptor, is a 90 kDa 7TM catecholamine receptor with selectivity for epinephrine and norepinephrine. Formation of homooligomers and heterooligomers with ADRA1A enables receptor internalization following ligand binding. ADRA1B functions in diverse settings including vasoconstriction and myocardial contractility, neuronal dopaminergic responses, dendritic cell migration and inflammatory responses, and neuroendocrine regulation of fertility. Human ADRA1B shares 95% amino acid sequence identity with mouse and rat ADRA1B.

• Long Name:

  Alpha-1B Adrenergic Receptor

• Entrez Gene IDs:

  147 (Human); 11548 (Mouse); 24173 (Rat)

• Alternate Names:

  a-1B AdrenergicR; ADRA1; ADRA1B; adrenergic, alpha-1B-, receptor; alpha-1B Adrenergic R; alpha-1B adrenergic receptor; alpha1B AdrenergicR; Alpha-1B adrenoceptor; Alpha-1B adrenoreceptor; alpha-1B-adrenergic receptor; ALPHA1BAR