• Species Reactivity

  Human

• Specificity

  Detects human Orexin R2/HCRTR2. Stains human Orexin R2/HCRTR2 transfectants but not irrelevant transfectants.

• Source

  Monoclonal Mouse IgG2a Clone # 456738

• Immunogen

  NS0 mouse myeloma cell line transfected with human Orexin R2/HCRTR2    
  Met1-Trp444    
  Accession # AAC39602

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 750

Applications

• Recommended    
  Concentration

  Sample

• Intracellular Staining by Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  A172 human glioblastoma cell line and HEK293 human embryonic kidney cell line transfected with human Orexin R2/HCRTR2 and eGFP were fixed with Flow Cytometry Fixation Buffer (Catalog # ) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # )

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Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Orexin R2/HCRTR2

Hypocretin receptor 2 (HCRTR2); also known as orexin receptor 2 or OX2R is a 40 kDa 7-transmembrane G-protein-coupled glycoprotein that is a high affinity receptor for orexins A and B (hypocretins 1 and 2). In mouse brain, engagement of HCRTRs promotes wakefulness, such that absence of either orexins or their receptors creates a narcolepsy-like state. It also influences reward circuits involving food or addictive drugs. The extracellular portions of human HCRTR2 share 92% and 93% aa identity with corresponding portions of mouse and rat HCRTR2, respectively.

• Long Name:

  Hypocretin Receptor Type 2

• Entrez Gene IDs:

  3062 (Human); 387285 (Mouse); 25605 (Rat)

• Alternate Names:

  HCRTR2; hypocretin (orexin) receptor 2; Hypocretin receptor type 2; Orexin R2; Orexin Receptor 2; orexin receptor type 2; OrexinR2; OX2R; Ox-2-R; ox2-R