• Species Reactivity

  Human

• Specificity

  Detects human LTBP-1 from the large latent TGF-beta 1 complex.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 35409

• Purification

  Protein A or G purified from ascites

• Immunogen

  Human platelet-derived large latent TGF-beta 1 complex

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Endotoxin Level

  <0.10 EU per 1 μg of the antibody by the LAL method.  

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Large latent TGF-beta complex from human platelets    
  under non-reducing conditions only

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• Immunoaffinity Purification

  This antibody can be used to affinity purify the large latent TGF-beta complex (containing both the 230 and 195 kDa binding protein) and will also pull out “free” binding protein that is not associated with the small latent TGF-beta.

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Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Latent TGF-beta bp1

TGF-beta  is synthesized as high molecular weight latent complexes. In platelets, the large latent TGF-beta 1 complex is composed of three components: mature TGF-beta 1 dimer; latency associated peptide; and a latent TGF-beta binding protein (LTBP) (1-3).The cDNAs for four binding proteins (LTBP-1, -2, -3 and -4) have now been cloned (4-5).

• References:

1. Miyazono, K. et al. (1991) EMBO J. 10:1091.

2. Moren, A. et al. (1994) J. Biol. Chem. 269:32469.

3. Yin, W. et al. (1995) J. Biol. Chem. 270:10147.

4. Kanzaki, T. et al. (1990) Cell 61:1051.

5. Saharinen, J. et al. (1998) J. Biol. Chem. 273:18459.

• Entrez Gene IDs:

  4052 (Human)

• Alternate Names:

  Latent TGFbeta bp1; Latent TGF-beta bp1; latent transforming growth factor beta binding protein 1; latent-transforming growth factor beta-binding protein 1; LTBP1; LTBP-1; MGC163161; TGF-beta1-BP-1; Transforming growth factor beta-1-binding protein 1