• Purity

  >95%, by SDS-PAGE with silver staining

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR1. The ED    ₅₀ for this effect is 0.5-3 ng/mL.

• Source

  E. coli-derived Met26-Asn92

• Accession #

• N-terminal Sequence    
  Analysis

  Met26

• Predicted Molecular Mass

  8 kDa

• SDS-PAGE

  12 kDa, reducing conditions

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: CCL4/MIP-1 beta

CCL4, also known as macrophage inflammatory protein 1 beta (MIP-1 beta ), is a 12 kDa beta chemokine that is secreted at sites of inflammation by activated leukocytes, lymphocytes, vascular endothelial cells, and pulmonary smooth muscle cells (1, 2). CCL4 attracts a variety of immune cells to sites of microbial infection as well as to other pathologic inflammation such as allergic asthma and ischemic myocardium (3-8). A CCL4 deficiency in mice promotes the development of autoantibodies, possibly as a result of compromised regulatory T cell recruitment (6). CCL4 is secreted from activated monocytes as a heterodimer with CCL3/MIP-1 alpha (9). The first two N-terminal amino acids can be cleaved from human CCL4 by CD26/DPPIV (10, 11). Both the full length and truncated forms exert biological activity through CCR5, and the truncated form additionally interacts with CCR1 and CCR2 (10). This recombinant protein corresponds to the truncated form of human CCL4.  In humans, the ability of CCL4 to bind CCR5 inhibits the cellular entry of M-tropic HIV-1 which utilizes CCR5 as a coreceptor (2). Both forms of CCL4 block HIV-1 infection of T cells by inducing the down-regulation of CCR5 (10). Mature human CCL4 shares 77% and 80% aa sequence identity with mouse and rat CCL4, respectively.

• References:

1. Rot, A. and U.H. von Andrian (2004) Annu. Rev. Immunol. 22:891.

2. Menten, P. et al. (2002) Cytokine Growth Factor Rev. 13:455.

3. Sun, X. et al. (2006) Infec. Immun. 74:5943.

4. Bisset, L.R. and Schmid-Grendelmeier, P. (2005) Curr. Opin. Pulm. Med. 11:35.

5. Frangogiannis, N.G. (2004) Inflamm. Res. 53:585.

6. Bystry, R.S. et al. (2001) Nat. Immunol. 2:1126.

7. Oliveira, S.H.P. et al. (2002) J. Leukoc. Biol. 71:1019.

8. Schall, T.J. et al. (1993) J. Exp. Med. 177:1821.

9. Guan, E. et al. (2001) J. Biol. Chem. 276:12404.

10. Guan, E. et al. (2002) J. Biol. Chem. 277:32348.

11. Guan, E. et al. (2004) J. Cell. Biochem. 92:53.

• Entrez Gene IDs:

  6351 (Human); 20303 (Mouse); 116637 (Rat); 448786 (Canine)

• Alternate Names:

  ACT2; ACT-2; AT744.1; C-C motif chemokine 4; CCL4; chemokine (C-C motif) ligand 4; Exodus-3; G-26 T-lymphocyte-secreted protein; G-26; HC21; LAG1; LAG-1; Lymphocyte activation gene 1 protein; Macrophage inflammatory protein 1-beta; MIP1 beta; MIP-1 beta; MIP1B; MIP1B1; MIP-1-beta; MIP-1-beta(1-69); PAT 744; Protein H400; SCYA2; SCYA4; secreted protein G-26; SIS-gamma; small inducible cytokine A4 (homologous to mouse Mip-1b); Small-inducible cytokine A4; T-cell activation protein 2