• Species Reactivity

  Human

• Specificity

  Detects human ADAMTS13 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) ADAMTS1, rhADAMTS4, rhADAMTS5, or rhADAMTS-L1.2 is observed.

• Source

  Monoclonal Mouse IgG1 Clone # 442323

• Immunogen

  Chinese hamster ovary cell line CHO-derived recombinant human ADAMTS13 isoform 1    
  Gln34-Trp688 (predicted)    
  Accession # Q76LX8

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 750

Applications

• Recommended    
  Concentration

  Sample

• Intracellular Staining by Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  HUVEC human umbilical vein endothelial cells fixed with paraformaldehyde and permeabilized with saponin

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: ADAMTS13

ADAMTS13 (a disintegrin and metalloproteinase with thrombospondin motifs 13) is a secreted multi-domain zinc protease primarily produced by the liver. In the circulation, it is responsible for cleaving von Willebrand factor (vWF) between Tyr842 and Met843. Defects in ADAMTS13 activity result in the generation of ultra large vWF, leading to thrombotic thrombocytopenic purpura (TTP). The amino acid sequence of human ADAMTS13 in the region of aa 34 to 688 is 76% identical to that of mouse/rat.

• Long Name:

  A Disintegrin-like and Metalloproteinase Domain with Thrombospondin Motifs 13

• Entrez Gene IDs:

  11093 (Human); 279028 (Mouse); 362091 (Rat)

• Alternate Names:

  A disintegrin and metalloproteinase with thrombospondin motifs 13; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 13; ADAM metallopeptidase with thrombospondin type 1 motif, 13; ADAMTS13; ADAM-TS13; ADAMTS-13; DKFZp434C2322; EC 3.4.24.14; EC 3.4.24.82; EC 3.4.24.87; FLJ42993; MGC118899; MGC118900; TTP; TTPADAM-TS 13; vWF-cleaving protease; vWF-CP; vWF-CPC9orf8; VWFCPvon Willebrand factor-cleaving protease