• Species Reactivity

  Human

• Specificity

  Detects human PKC theta. This antibody also detects recombinant human PKC theta, but does not detect any other recombinant human PKC family member.

• Source

  Monoclonal Mouse IgG2a Clone # 453416

• Immunogen

  E. coli-derived recombinant human PKC theta    
  His580-Ser706    
  Accession # Q04759

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 750

Applications

• Recommended    
  Concentration

  Sample

• Intracellular Staining by Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  Jurkat human acute T cell leukemia cell line fixed with paraformaldehyde and permeabilized with methanol

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: PKC theta

PKC theta (PRKCQ) is a calcium-independent phospholipid-dependent serine and threonine kinase that belongs to the novel PKC subfamily. PKC theta plays a critical role in several aspects of T cell biology, and knockout mice develop autoimmune disease. PKC theta functions through the activation of NF kappa B and AP1 transcription factors.

• Long Name:

  Protein Kinase C theta

• Entrez Gene IDs:

  5588 (Human); 18761 (Mouse); 85420 (Rat)

• Alternate Names:

  EC 2.7.11; EC 2.7.11.13; MGC126514; MGC141919; nPKC-theta; PKC theta; PRKCQ; PRKCT; protein kinase C theta type; protein kinase C, theta