• Species Reactivity

  Porcine

• Specificity

  Detects porcine IL-6 in direct ELISAs and Western blots. In direct ELISAs and Western blots, partial cross‑reactivity with recombinant human, mouse, canine, equine, and feline IL-6 is observed, and no cross‑reactivity with recombinant human (rh) CLC, rhIL-11, rhCNTF, rhCT-1, rhLIF, or rhOSM is observed.

• Source

  Monoclonal Mouse IgG2b Clone # 90613

• Immunogen

  E. coli-derived recombinant porcine IL-6    
  Pro29-Met212    
  Accession # P26893

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 750

Applications

• Recommended    
  Concentration

  Sample

• Intracellular Staining by Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  Porcine peripheral blood mononuclear cells treated with LPS, fixed with paraformaldehyde, and permeabilized with saponin

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-6

Interleukin 6 (IL-6) is a pleiotropic alpha -helical cytokine that plays important roles in acute phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6 activity is central to the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. It is secreted by multiple cell types as a 22 kDa‑28 kDa phosphorylated and variably glycosylated molecule (1‑4). Mature porcine IL-6 is 183 amino acids (aa) in length and shares 61%, 42%, and 42% aa sequence identity with human, mouse, and rat IL-6 (5). IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R, triggering IL-6 R association with gp130 and gp130 dimerization (6). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (7). Soluble forms of IL-6 R are generated by both alternate splicing and proteolytic cleavage (3). In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R elicit responses from gp130-expressing cells that lack cell surface IL‑6 R (3). Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous while that of IL-6 R is predominantly restricted to hepatocytes, leukocytes, and lymphocytes (3). Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R but not from other cytokines that utilize gp130 as a coreceptor (4, 8).

• References:

1. Van Snick, J. (1990) Annu. Rev. Immunol. 8:253.

2. Hodge, D.R. et al. (2005) Eur. J. Cancer 41:2502.

3. Jones, S.A. (2005) J. Immunol. 175:3468.

4. Rose-John, S. et al. (2006) J. Leukoc. Biol. 80:227.

5. Mathialagan, N. et al. (1992) Mol. Reprod. Dev. 32:324.

6. Murakami, M. et al. (1993) Science 260:1808.

7. Muller-Newen, G. (2003) Sci. STKE 2003:PE40.

8. Mitsuyama, K. et al. (2006) Clin. Exp. Immunol. 143:125.

• Long Name:

  Interleukin 6

• Entrez Gene IDs:

  3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 100034196 (Equine); 493687 (Feline); 100008733 (Rabbit)

• Alternate Names:

  B cell stimulatory factor-2; B-cell differentiation factor; BSF-2; BSF2CTL differentiation factor; CDF; HGFHSFIFNB2Hybridoma growth factor; IFN-beta-2; IL6; IL-6; IL-6B-cell stimulatory factor 2; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A