• Species Reactivity

  Mouse

• Specificity

  Detects mouse ALCAM/CD166 in Western blots and direct ELISAs. In Western blots, shows 100% cross-reactivity with recombinant human (rh) ALCAM and no cross-reactivity with rhBCAM, rhEpCAM, recombinant mouse (rm) MAdCAM-1, rhMCAM, rmNCAM-L1, rmOCAM, or rmTROP-2.

• Source

  Monoclonal Rat IgG2a Clone # 200622

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse ALCAM/CD166    
  Trp28-Lys527    
  Accession # AAC06342

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 350

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  Mouse splenocytes treated with PHA

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: ALCAM/CD166

ALCAM, activated leukocyte cell adhesion molecule, is a type I membrane glycoprotein and a member of the immunoglobulin supergene family. It is also known as CD166, MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. ALCAM is expressed on thymic epithelial cells, activated B and T cells, and monocytes. ALCAM can bind itself homotypically and is also capable of binding CD6, NgCAM, and other, as of yet, unidentified brain proteins. ALCAM/CD6 interaction may be involved in T cell development and T cell regulation. Additionally, ALCAM/CD6 and ALCAM/NgCAM interactions may play roles in the nervous system. ALCAM has also been observed to be upregulated on highly metastasizing melanoma cell lines and may play a role in tumor migration. ALCAM is a 583 amino acid (aa) protein consisting of a 27 aa signal peptide, a 500 aa extracellular domain, a 24 aa transmembrane domain and a 32 aa cytoplasmic domain. The extracellular domain of ALCAM contains 5 Ig-like domains of which the amino-terminal V1 domain is essential for ligand binding and ALCAM‑mediated cell aggregation (1‑4).

• References:

1. Bowen, M.A. et al. (1995) J. Exp. Med. 181:2213.

2. Aruffo, A. et al. (1997) Immunol. Today 18:498.

3. Degen, W.G. et al. (1998) Am. J. Pathol. 152:805.

4. Van Kempen, L. et al. (2001) J. Biol. Chem. 276:25783.

• Long Name:

  Activated Leukocyte Cell Adhesion Molecule

• Entrez Gene IDs:

  214 (Human); 11658 (Mouse)

• Alternate Names:

  activated leucocyte cell adhesion molecule; activated leukocyte cell adhesion moleculeFLJ38514; ALCAM; CD166 antigen; CD166; MEMDMGC71733