• Species Reactivity

  Mouse

• Specificity

  Detects mouse B220/CD45R.

• Source

  Monoclonal Rat IgG2a Clone # RA3-6B2

• Immunogen

  Abelson mouse leukemia virus-induced pre-B tumor cells

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 350

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  Mouse splenocytes

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: B220/CD45R

B220 is the mouse CD45 isoform CD45R, which is predominantly expressed on all B lymphocytes, including pro-, mature and activated B cells (1‑3). The level of B220 antigen expression on the B cell lineage is developmentally regulated (2‑4) and this antibody is commonly used as a B cell marker. RA3-6B2 has been reported to inhibit in vivo B cell responses (5, 6).

• References:

1. Coffman, R.L. (1982) Immuno. Rev. 69:5.

2. Hardy, R.R. et al. (1991) J. Exp. Med. 173:1213.

3. Hathcock, K.S. et al. (1992) J. Immunol. 149:2286.

4. Allman, D.M. et al. (1992) J. Immunol. 149:2533.

5. Asensi, V.K. et al. (1989) Immunology 68:204.

6. Domiati-Saad, R. et al. (1993) J. Immunol. 151:5936.

• Long Name:

  Cluster of Differentiation 45

• Entrez Gene IDs:

  5788 (Human); 19264 (Mouse); 24699 (Rat)

• Alternate Names:

  B220; CD45 antigen; CD45R; EC 3.1.3.48; L-CA; LY5; protein tyrosine phosphatase, receptor type, C; receptor-type tyrosine-protein phosphatase C; T200 glycoprotein; T200 leukocyte common antigen; T200receptor type, c polypeptide