• Species Reactivity

  Mouse

• Specificity

  Detects mouse NKG2D in direct ELISAs and Western blots. Does not cross-react with recombinant human NKG2D.

• Source

  Monoclonal Rat IgG2b Clone # 191004

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse NKG2D    
  Phe94-Val232    
  Accession # O54709

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 750

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  NK1.1    ⁺ mouse splenic NK cell

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: NKG2D/CD314

NKG2D is a type II transmembrane protein with an extracellular C-type lectin-like domain. It occurs as a disulfide-linked homodimer that associates with the transmembrane DAP10 (DNAX-activator protein 10) adapter protein to deliver an activating signal. This protein shares approximately 25% amino acid sequence identity with a number of other type II lectin-like proteins that are encoded by genes within the natural killer complex on mouse chromosome 6. NKG2D is expressed on NK cells, where it functions as an activating receptor to trigger cytolytic activity and cytokine secretion, and on some T cell subsets, where it acts as a co‑stimulatory receptor complementing T cell receptor signaling. Several ligands have now been identified for mouse NKG2D including H60 and Rae 1 alpha, beta, gamma, δ, and epsilon. All of these ligands are cell-surface proteins distantly related to MHC class I. However, they do not bind peptide or associate with beta 2-microglobulin. Ligand expression is up-regulated in many transformed cell lines and also during conditions of stress such as heat shock or viral infection. In vivo, tumor models demonstrate that NKG2D functions in anti-tumor surveillance (1-5).

• References:

1. Bauer, S. et al. (1999) Science 285:727.

2. Wu, J. et al. (1999) Science 285:730.

3. Diefenbach, A. et al. (2001) Nature 413:165.

4. Vivier, E. et al. (2002) Curr. Opin. Immunol. 14:306.

5. NKG2D and its Ligands; www.RnDSystems.com.

• Long Name:

  Natural Killer G2D

• Entrez Gene IDs:

  22914 (Human); 27007 (Mouse)

• Alternate Names:

  CD314 antigen; CD314; D12S2489E; FLJ17759; FLJ75772; Killer cell lectin-like receptor subfamily K member 1; killer cell lectin-like receptor subfamily K, member 1; KLR; KLRK1; NK cell receptor D; NKG2-D type II integral membrane protein; NKG2D; NKG2-D; NKG2-D-activating NK receptor; NKG2DDNA segment on chromosome 12 (unique) 2489 expressed sequence