• Assay Type

  Solid Phase Sandwich ELISA

• Format

  96-well strip plate

• Sample Type & Volume Required

  100 µL

• Range

  156.00 - 10,000 pg/mL

• Sufficient Materials

  For five 96-well plates*

• Specificity

  Please see the

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Ancillary Reagent Kit Available  

DY008, DuoSet ELISA Ancillary Reagent Kit 2 -

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and Recombinant

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Product Features

• Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time

• Development protocols are provided to guide further assay optimization

• Assay can be customized to your specific needs

• Economical alternative to complete kits

Kit Content

• Capture Antibody

• Detection Antibody

• Recombinant Standard

• Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

  PBS: (Catalog # ), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na  ₂HPO  ₄, 1.5 mM KH  ₂PO  ₄, pH 7.2 - 7.4, 0.2 µm filtered  
 
  Wash Buffer: (Catalog # ), or equivalent  
 
  Reagent Diluent*  
 
  Blocking Buffer*  
 
  Substrate Solution: 1:1 mixture of Color Reagent A (H  ₂O  ₂) and Color Reagent B (Tetramethylbenzidine) (Catalog # )  
 
  Stop Solution: 2 N H  ₂SO  ₄ (Catalog # )  
 
  Microplates: R&D Systems (Catalog # ), or equivalent  
 
  Plate Sealers: ELISA Plate Sealers (Catalog # ), or equivalent  
 

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Preparation and Storage

• Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: MIS RII

Müllerian inhibiting substance receptor II (MIS RII) is a type II TGF-beta superfamily receptor that forms a heteromeric complex with various type I receptors to mediate MIS signaling. Mature MIS RII has a cysteine-rich, glycosylated, extracellular domain, and a cytoplasmic region containing a serine/threonine kinase domain. Rat MIS RII shares 95% and 82% aa sequence identity with its mouse and human orthologs, respectively. Embryonically, it is expressed in mesenchymal cells surrounding the Müllerian ducts. Postnatally, it is expressed in uterine tissues and Leydig cells, showing coexpression with MIS in the testicular Sertoli and ovarian granulosa cells.

• Entrez Gene IDs:

  269 (Human); 110542 (Mouse); 29530 (Rat);

• Long Name:

  Mullerian-Inhibiting Substance Type II Receptor

• Aliases:

  AMH type II receptor; AMHR2; AMHREC 2.7.11.30; AMHRII; C14; MIS RII; MISR2; MISRII; MISRIIMIS type II receptor; MRII; Muellerian hormone type II receptor; Muellerian hormone type-2 receptor; Mullerian hormone receptor, type II; Mullerian inhibiting substance type II receptor