• Species Reactivity

  Human

• Specificity

  Detects human NT-3 in ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant human (rh) BDNF, rhCNTF, rhGDNF,    
  rh beta ‑NGF, and rhNT-4 is observed.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 41512

• Purification

  Protein A or G purified from ascites

• Immunogen

  S. frugiperda insect ovarian cell line     Sf 21-derived recombinant human NT-3    
  Tyr139-Thr257    
  Accession # P20783

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Endotoxin Level

  <0.10 EU per 1 μg of the antibody by the LAL method.  

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Human NT-3 Sandwich Immunoassay

  Reagent    

• ELISA Capture (Matched Antibody Pair)

  2-8 µg/mL 

  Human NT‑3 Antibody (Catalog # )

• ELISA Detection (Matched Antibody Pair)

  0.1-0.4 µg/mL 

  Human NT‑3 Biotinylated Antibody (Catalog # )

• ELISA Standard

   

  Recombinant Human NT-3 Protein (Catalog # )

• 
  

• Neutralization

  Measured by its ability to neutralize NT‑3-induced proliferation in BaF‑TrKB‑BD mouse pro‑B cell line transfected with TrkB. The Neutralization Dose (ND     ₅₀) is typically 0.1-0.5 µg/mL in the presence of 100 ng/mL Recombinant Human NT‑3.

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NT-3

Neurotrophin-3 (NT-3) is a member of the NGF family of neurotrophic factors (also named neurotrophins) that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous systems. The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT-3, and NT-4/5. These secreted cytokines are synthesized as prepropeptides that are proteolytically processed to generate the mature proteins. All neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds and all share approximately 55% sequence identity at the amino acid level. Similarly to NGF, bioactive NT-3 is predicted to be a non-covalently linked homodimer.

The NT-3 cDNA encodes a 257 amino acid residue precursor protein with a signal peptide and a proprotein that are cleaved to yield the 119 amino acid residue mature NT-3. The amino acid sequence of mature NT-3 is identical in human, mouse and rat. NT-3 transcripts have been detected in the cerebellum, hippocampus, placenta, heart, skin, and skeletal muscle. NT-3 primarily activates the TrkC tyrosine kinase receptor. In addition, NT-3 can activate Trk and TrkB kinase receptors in certain cell systems. NT-3 can also bind with low affinity to the low affinity NGF receptor.

• References:

1. Eide, F.F. et al. (1993) Exp. Neurol. 121:200.

2. Snider, W.D. (1994) Cell 77:627.

3. Barbacid, M. (1994) J. Neurobiol. 25:1386.

• Long Name:

  Neurotrophin 3

• Entrez Gene IDs:

  4908 (Human)

• Alternate Names:

  3-nucleotidase; NT3; NT-3; NTF3