• Species Reactivity

  Human

• Specificity

  Detects human CXCR1/IL-8 RA transfectants but not the parental cell line. It does not cross-react with human CXCR2.

• Source

  Monoclonal Mouse IgG    _2A Clone # 42705

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  NS0 mouse myeloma cell line transfected with human CXCR1/IL-8 RA    
  Met1-Leu350    
  Accession # AAA59159

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Endotoxin Level

  <0.10 EU per 1 μg of the antibody by the LAL method.  

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25 µg/10    ⁶ cells

  See below

• 
  

• Immunohistochemistry

  8-25 µg/mL

  See below

• 
  

• CyTOF-reported

  Cheng, Y.     et al. (2016) J. Immunol.     196: 924. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

• 
  

• Neutralization

  Measured by its ability to neutralize CXCL8/IL‑8-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR1. The Neutralization Dose (ND    ₅₀) is typically 0.4-2.0 µg/mL in the presence of 1 ng/mL Recombinant Human CXCL8/IL‑8.

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCR1/IL-8 RA

The human C-X-C chemokine IL-8 is a potent neutrophil chemotactic and activating factor. Two distinct G protein-linked cell surface receptors, known as IL-8 RA (type I or CXCR1) and IL-8 RB (type II or CXCR2), can interact with the IL-8 molecule. These two receptors share 77% amino acid homology. CXCR1 expression has been documented on neutrophils, monocytes, and a small population of T cells.

• Long Name:

  Interleukin 8 Receptor A

• Entrez Gene IDs:

  3577 (Human); 227288 (Mouse); 54258 (Rat)

• Alternate Names:

  CD128; CD181 antigen; CD181; CDw128aC-C; chemokine (C-X-C motif) receptor 1; CKR-1; CMKAR1; C-X-C chemokine receptor type 1; CXCR1; CXC-R1; CXCR-1; High affinity interleukin-8 receptor A; IL-8 receptor type 1; IL-8R A; IL8R1; IL8RA; IL-8RA; IL8RAC-C-CKR-1; IL8RBA; interleukin 8 receptor, alpha; interleukin-8 receptor type 1; interleukin-8 receptor type A