• Species Reactivity

  Human

• Specificity

  Captures human Leptin/OB in sandwich ELISAs. In this format, this antibody shows less than 0.1% cross-reactivity with recombinant mouse (rm) Leptin/OB and rrLeptin/OB.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 44802

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human Leptin/OB    
  Val22-Cys167    
  Accession # P41159

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Endotoxin Level

  <0.10 EU per 1 μg of the antibody by the LAL method.  

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Human Leptin/OB Sandwich Immunoassay

  Reagent    

• ELISA Capture (Matched Antibody Pair)

  2-8 µg/mL 

  Human Leptin/OB Antibody (Catalog # )

• ELISA Detection (Matched Antibody Pair)

  0.5-2.0 µg/mL 

  Human Leptin/OB Biotinylated Antibody (Catalog # )

• ELISA Standard

   

  Recombinant Human Leptin Protein, CF (Catalog # )

• 
  

• Neutralization

  Measured by its ability to neutralize Leptin/OB-induced proliferation in the BaF3 mouse pro‑B cell line transfected with human Leptin R. The Neutralization Dose (ND     ₅₀) is typically 0.4-1.6 µg/mL in the presence of 7.5 ng/mL Recombinant Human Leptin/OB.

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Leptin/OB

Leptin is a protein product of the mouse obese gene. Mice with mutations in the obese gene that block the sythesis of Leptin have been found to be obese, diabetic and to have reduced activity, metabolism and body temperature. cDNA clones encoding Leptin have been isolated from human, simian, mouse and rat cells. Human Leptin shares approximately 84% sequence identity with the mouse protein. Human Leptin cDNA encodes a 167 amino acid residue protein with a 21 amino acid residue signal sequence that is cleaved to yield the 146 amino acid residue mature protein. The expression of Leptin mRNA has been shown to be restricted to adipose tissue.

A high-affinity receptor for Leptin (OB-R) with homology to gp130 and the G-CSF receptor has been cloned. OB-R mRNA has been shown to be expressed in the choroid plexus and in the hypothalamus. OB-R has also been identified as an isoform of B219, a sequence that is expressed in at least four isoforms in very primitive hematopoietic cell populations and in a variety of lymphohematopoietic cell lines (1-3). The roles of leptin in body weight regulation, hematopoiesis and reproduction continue to be investigated.

• References:

1. Considine, R. and J. Caro (1996) Clinical Chemistry 42:843.

2. Tartaglia, L.A. et al. (1995) Cell 83:1263.

3. Cioffi, J.A. et al. (1996) Nature Medicine 2:585.

• Entrez Gene IDs:

  3952 (Human); 16846 (Mouse); 25608 (Rat)

• Alternate Names:

  FLJ94114; LEP; leptin (murine obesity homolog); leptin (obesity homolog, mouse); Leptin; OB; Obese protein; obese, mouse, homolog of; Obesity factor; OBOBS