• Specificity

  Detects TGF-beta 3 from multiple species in ELISAs and Western blots. In ELISAs, does not cross-react with recombinant human (rh) TGF-beta 1or rhTGF-beta 2.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 44922

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  S. frugiperda insect ovarian cell line     Sf 21-derived recombinant human TGF‑ beta 3    
  Ala301-Ser412 (Tyr340Phe)    
  Accession # NP_003230.1

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Human TGF-beta 3 (Catalog # )    
  under non-reducing conditions only

• 
  

• Immunohistochemistry

  8-25 µg/mL

  See below

• 
  

• Human TGF-beta 3 Sandwich Immunoassay

  Reagent    

• ELISA Capture (Matched Antibody Pair)

  2-8 µg/mL 

  TGF‑ beta 3 Antibody (Catalog # )

• ELISA Detection (Matched Antibody Pair)

  0.1-0.4 µg/mL 

  TGF‑ beta 3 Biotinylated Antibody (Catalog # )

• ELISA Standard

   

  Recombinant Human TGF-beta 3 Protein (Catalog # )

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TGF-beta 3

The active form of TGF-beta 3 is a disulfide-linked homodimer which is generated by activation of the latent TGF-beta 3 complex. TGF-beta s are multifunctional cytokines that activate or repress multiple genes in a wide variety of cell types. Depending on the cell context, TGF-beta can control many cellular processes including growth, morphogenesis, and apoptosis.

• Long Name:

  Transforming Growth Factor beta 3

• Entrez Gene IDs:

  7043 (Human); 21809 (Mouse); 25717 (Rat)

• Alternate Names:

  ARVD; FLJ16571; TGFB3; TGFbeta 3; TGF-beta 3; TGF-beta3; TGF-beta-3; transforming growth factor beta-3; transforming growth factor, beta 3