• Species Reactivity

  Human, Mouse

• Specificity

  Detects human and mouse FGF-8 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross-reactivity with recombinant human  (rh) FGF-5, rhFGF-7, and rhFGF-9 is observed.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 47109

• Purification

  Protein A or G purified from ascites

• Immunogen

  E. coli-derived recombinant mouse FGF-8b    
  Gln23-Arg215    
  Accession # NP_001159834

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Endotoxin Level

  <0.10 EU per 1 μg of the antibody by the LAL method.  

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Human FGF-8a (Catalog # )    
  Recombinant Mouse FG-8b (Catalog # )    
  Recombinant Mouse FGF-8c (Catalog # )    
  Recombinant Human FGF-8e (Catalog # )    
  Recombinant Human FGF-8f (Catalog # )

• 
  

• Immunohistochemistry

  8-25 µg/mL

  See below

• 
  

• Neutralization

  Measured by its ability to neutralize FGF‑8-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line. Rizzino, A.     et al. (1988) Cancer Res.     48:4266. The Neutralization Dose (ND    ₅₀) is typically 0.25-0.75 µg/mL in the presence of 125 ng/mL Recombinant Mouse FGF‑8 c Isoform and 0.1 µg/mL heparin.

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF-8

FGF-8 is a member of the fibroblast growth factor family that was originally discovered as a growth factor essential for the androgen-dependent growth of mouse mammary carcinoma cells (1-3). Alternate splicing of mouse FGF-8 mRNA generates eight secreted isoforms, designated a-h, but only FGF-8a, b, e and f exist in humans (4). FGF-8 contains a 22 amino acid (aa) signal sequence, an N‑terminal domain that varies according to the isoform (30 aa for FGF-8b; 20 aa for the shortest, FGF-8a), a 125 aa FGF domain and a 37 aa proline‑rich C‑terminal sequence. The FGF domain of FGF-8 shares the most aa identity with FGF17 (75%) and FGF-18 (67%), and the three form an FGF subfamily (2). Mouse FGF-8b shares 100% aa identity with human FGF-8b. FGF-8 is widely expressed during embryogenesis, and mediates epithelial-mesenchymal transitions. It plays an organizing and inducing role during gastrulation, and regulates patterning of the midbrain/hindbrain, eye, ear, limbs and heart in the embryo (2, 5 - 8). The isoforms may play different roles in development. FGF-8b shows the strongest receptor affinity and oncogenic transforming capacity although FGF-8a and FGF-8e are also transforming and have been found in human prostate, breast or ovarian tumors (1, 5, 9-12). FGF-8 shows limited expression in the normal adult, but low levels are found in the reproductive and genitourinary tract, peripheral leukocytes and bone marrow hematopoietic cells (3, 9, 13).

• References:

1. Mattila, M.M. and P.L. Harkonen (2007) Cytokine Growth Factor Rev. 18:257.

2. Reuss, B. and O. von Bohlen und Halbach (2003) Cell Tiss. Res. 313:139.

3. Tanaka, A. et al. (1992) Proc. Natl. Acad. Sci. USA 89:8928.

4. Gemel, J. et al. (1996) Genomics 35:253.

5. Olsen, S.K. et al. (2006) Genes Dev. 20:185.

6. Crossley, P.H. et al. (1996) Cell, 84:127.

7. Heikinheimo, M. et al. (1994) Mech. Dev. 48:129.

8. Sun, X. et al. (1999) Genes Dev. 13:1834.

9. Ghosh, A.K. et al. (1996) Cell Growth Differ. 7:1425.

10. Mattila, M.M. et al. (2001) Oncogene 20:2791.

11. Valve, E. et al. (2000) Int. J. Cancer 88:718.

12. Valve, E.M. et al. (2001) Lab. Invest. 81:815.

13. Nezu, M. et al. (2005) Biochem. Biophys. Res. Commun. 335:843.

• Long Name:

  Fibroblast Growth Factor 8

• Entrez Gene IDs:

  2253 (Human); 14179 (Mouse); 29349 (Rat)

• Alternate Names:

  AIGF; AIGFKAL6; Androgen-induced growth factor; FGF8; FGF-8; fibroblast growth factor 8 (androgen-induced); fibroblast growth factor 8; HBGF-8; Heparin-binding growth factor 8; MGC149376