• Species Reactivity

  Mouse

• Specificity

  Stains mouse CD9-transfected cells but not irrelevant transfecants.

• Source

  Monoclonal Rat IgG    _2A Clone # 479608

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  NS0 mouse myeloma cell line transfected with mouse CD9    
  Pro2-Val226    
  Accession # P40240

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  2.5 µg/10    ⁶ cells

  Mouse D3 cell line

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• Immunohistochemistry

  8-25 µg/mL

  Perfusion fixed frozen sections of mouse kidney and thymus

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• CyTOF-ready

  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD9

CD9, also known as motility-related protein-1 (MRP-1), is a 24 kDa glycoprotein of the tetraspanin family that is expressed by a variety of hematopoietic and epithelial cells. It forms homotypic and heterotypic associations with other tetraspanin proteins, some integrins and MHC class II proteins. CD9 has been shown to modulate cellular adhesion, migration and proliferation. The extracellular portions of mouse CD9 share 81% and 88% amino acid identity with corresponding regions of human and rat CD9, respectively.

• Entrez Gene IDs:

  928 (Human); 12527 (Mouse)

• Alternate Names:

  BA2; BA-2/p24 antigen; BTCC-1; CD9 antigen; CD9 molecule; CD9; Cell growth-inhibiting gene 2 protein; DRAP-27; Leukocyte antigen MIC3; MIC3; motility related protein-1,5H9 antigen; Motility-related protein; MRP-1FLJ99568; p24; tetraspanin-29; TSPAN29; TSPAN-29; TSPAN29MIC3CD9 antigen (p24)