• Species Reactivity

  Human

• Specificity

  Detects human IL-3 in ELISAs and Western blots. In sandwich ELISAs, no significant cross-reactivity or interference with recombinant human (rh) IL-1 alpha, rhIL-1 beta, recombinant mouse (rm) IL-1 beta, rhIL-2, rhIL-3 R alpha, rhIL-3 R beta, rmIL-3, rhIL-4, rmIL-4, rmIL-5, rhIL-6, rmIL-6, rhIL-7, rmIL-7, rhIL-8, bovine FGF acidic, bovine FGF basic, rhG-CSF, rhGM-CSF, rmGM-CSF, rhLIF, hPDGF, porcine PDGF, human TGF‑ beta 1, porcine TGF‑ beta 1, rhTGF‑ beta 1, porcine TGF‑ beta 2, porcine TGF‑ beta 1.2, rhTNF-alpha, or rhTNF-beta was observed. Does not neutralize the biological activity of rmIL‑3.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 4815

• Purification

  Protein A or G purified from ascites

• Immunogen

  E. coli-derived recombinant human IL-3    
  Ala20-Phe152    
  Accession # P08700

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Endotoxin Level

  <0.10 EU per 1 μg of the antibody by the LAL method.  

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Human IL-3 Sandwich Immunoassay

  Reagent    

• ELISA Capture (Matched Antibody Pair)

  2-8 µg/mL 

  Human IL‑3 Antibody (Catalog # )

• ELISA Detection (Matched Antibody Pair)

  0.1-0.4 µg/mL 

  Human IL‑3 Biotinylated Antibody (Catalog # )

• ELISA Standard

   

  Recombinant Human IL-3 Protein (Catalog # )

• 
  

• Neutralization

  Measured by its ability to neutralize IL‑3-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T.      et al. (1989) J. Cell Physiol.      140:323. The Neutralization Dose (ND     ₅₀) is typically 0.1-0.3 µg/mL in the presence of 1.25 ng/mL Recombinant Human IL‑3.

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-3

Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. In addition, IL-3 also affects the functional activity of mature mast cells, basophils, eosinophils and macrophages. Because of its multiple functions and targets, it was originally studied under different names, including mast cell growth factor, P-cell stimulating factor, burst promoting activity, multi-colony stimulating factor, thy-1 inducing factor and WEHI-3 growth factor. In addition to activated T cells, other cell types such as human thymic epithelial cells, activated murine mast cells, murine keratinocytes and neurons/astrocytes can also produce IL-3. At the amino acid sequence level, mature human and murine IL-3 share only 29% sequence identity. Consistent with this lack of homology, IL-3 activity is highly species-specific and human IL-3 does not show activity on murine cells.

IL-3 exerts its biological activities through binding to specific cell surface receptors. The high affinity receptor responsible for IL-3 signaling is composed of at least two subunits, an IL-3 specific alpha chain which binds IL-3 with low affinity and a common beta chain that is shared by the IL-5 and GM-CSF high-affinity receptors. Although the beta chain itself does not bind IL-3, it confers high-affinity IL-3 binding in the presence of the  alpha chain. Receptors for IL-3 are present on bone marrow progenitors, macrophages, mast cells, eosinophils, megakaryocytes, basophils and various myeloid leukemic cells.

• Long Name:

  Interleukin 3

• Entrez Gene IDs:

  3562 (Human); 16187 (Mouse); 24495 (Rat)

• Alternate Names:

  Hematopoietic growth factor; IL3; IL-3; IL-3MGC79398; interleukin 3 (colony-stimulating factor, multiple); interleukin-3; Mast cell growth factor; mast-cell growth factor; MCGFMGC79399; MULTI-CSF; multilineage-colony-stimulating factor; Multipotential colony-stimulating factor; P-cell stimulating factor; P-cell-stimulating factor