• Species Reactivity

  Human

• Specificity

  Detects human CXCL9/MIG in ELISAs and Western blots. In ELISAs, does not cross-react with recombinant mouse (rm) CXCL9, recombinant human CXCL10.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 49106

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human CXCL9/MIG    
  Thr23-Thr125    
  Accession # Q07325

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Endotoxin Level

  <0.10 EU per 1 μg of the antibody by the LAL method.  

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Human CXCL9/MIG (Catalog # )    
  under non-reducing conditions only

• 
  

• CyTOF-ready

  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

• 
  

• Immunocytochemistry

  8-25 µg/mL

  See below

• 
  

• Intracellular Staining by Flow Cytometry

  2.5 µg/10    ⁶ cells

  THP-1 cells treated with Recombinant Human IFN‑ gamma (Catalog # ), fixed with paraformaldehyde, and permeabilized with saponin

• 
  

• Human CXCL9/MIG Sandwich Immunoassay

  Reagent    

• ELISA Capture (Matched Antibody Pair)

  2-8 µg/mL 

  Human CXCL9/MIG Antibody (Catalog # )

• ELISA Detection (Matched Antibody Pair)

  0.1-0.4 µg/mL 

  Human CXCL9/MIG Biotinylated Antibody (Catalog # )

• ELISA Standard

   

  Recombinant Human CXCL9/MIG Protein (Catalog # )

• 
  

• Neutralization

  Measured by its ability to neutralize CXCL9/MIG-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CXCR3. The Neutralization Dose (ND     ₅₀) is typically 4-20 µg/mL in the presence of 0.25 µg/mL Recombinant Human CXCL9/MIG.

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL9/MIG

CXCL9, a member of the alpha  subfamily of chemokines that lack the ELR domain, was initially identified as a lymphokine-activated gene in mouse macrophages. Human CXCL9 was subsequently cloned using mouse MIG cDNA as a probe. The CXCL9 gene is induced in macrophages and in primary glial cells of the central nervous system specifically in response to IFN-gamma. CXCL9 has been shown to be a chemoattractant for activated T-lymphocytes and TIL but not for neutrophils or monocytes. The human CXCL9 cDNA encodes a 125 amino acid (aa) residue precursor protein with a 22 aa residue signal peptide that is cleaved to yield a 103 aa residue mature protein. CXCL9 has an extended carboxy-terminus containing greater than 50% basic aa residues and is larger than most other chemokines. The carboxy-terminal residues of CXCL9 are prone to proteolytic cleavage resulting in size heterogeneity of natural and recombinant CXCL9. CXCL9 with large carboxy-terminal deletions have been shown to have diminished activity in the calcium flux assay. A chemokine receptor (CXCR3) specific for CXCL9 and IP-10 has been cloned and shown to be highly expressed in IL-2-activated T-lymphocytes. The E. coli-expressed CXCL9 preparations produced at R&D Systems have been shown to contain greater than 80% full length CXCL9.

• References:

1. Loetscher, M. et al. (1996) J. Exp. Med. 184:963.

2. Liao, F. et al. (1995) J. Exp. Med. 182:1301.

3. Vanguri, P. (1995) J. Neuroimmunol. 56:35.

• Entrez Gene IDs:

  4283 (Human); 17329 (Mouse); 246759 (Rat)

• Alternate Names:

  chemokine (C-X-C motif) ligand 9; CMK; crg-10; C-X-C motif chemokine 9; CXCL9; Gamma-interferon-induced monokine; Humig; MIG; MIGSmall-inducible cytokine B9; monokine induced by gamma interferon; SCYB9Monokine induced by interferon-gamma