• Species Reactivity

  Mouse

• Specificity

  Detects mouse CD39/ENTPD1 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human CD39 is observed and no cross-reactivity with recombinant mouse CD39L3 or recombinant human CD39L4 is observed.

• Source

  Monoclonal Rat IgG    ₁ Clone # 495826

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse CD39/ENTPD1    
  Thr38-Ile478    
  Accession # AAH11278

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Mouse CD39/ENTPD1 (Catalog # )

• 
  

• Flow Cytometry

  2.5 µg/10    ⁶ cells

  See below

• 
  

• Immunoprecipitation

  25 µg/mL

  Conditioned cell culture medium spiked with Recombinant Mouse CD39/ENTPD1 (Catalog # ),

• 
  

• CyTOF-ready

  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD39/ENTPD1

Ectonucleoside triphosphate diphosphohydrolase-1 (NTPDase-1) is an integral membrane protein with an extracellular active site. NTPDase-1 was originally described as CD39, a B lymphocyte cell surface marker (1), but it is also present on the surface of natural killer cells, T cells, and some endothelial cells (2). NTPDase-1 hydrolyzes the beta - and gamma  phosphate residues of nucleotides, preferring ATP as the substrate. Through its hydrolysis of extracellular nucleotides, NTPDase-1 plays a role in the regulation of purinergic signaling (3). NTPDase-1 is involved in the processes of thromboregulation and vascular inflammation (4). The administration of soluble NTPDase-1 may have therapeutic applications for the treatment of some vascular and transplantation-associated diseases (5).

• References:

1. Rowe, M. et al. (1982) Int. J. Cancer 29:373.

2. Kansas, G.S. et al. (1991) J. Immunol. 146:2235.

3. Kishore, B.K. et al. (2005) Am. J. Physiol. Renal Physiol. 288:F1032.

4. Marcus, A.J. et al. (2005) Semin. Thromb. Hemost. 31:234.

5. Robson, S.C. et al. (2005) Semin. Thromb. Hemost. 31:217.

• Long Name:

  Ecto-Nucleoside Triphosphate Diphosphohydrolase 1

• Entrez Gene IDs:

  953 (Human); 12495 (Mouse); 64519 (Rat); 102132860 (Cynomolgus Monkey)

• Alternate Names:

  ADPase; ATPDase; ATP-diphosphatase; CD39 antigen; CD39; CD39EC 3.6.1.5; DKFZp686D194; DKFZp686I093; EC 3.6.1; ecto-apyrase; Ecto-ATP diphosphohydrolase 1; Ecto-ATPase 1; Ecto-ATPDase 1; ectonucleoside triphosphate diphosphohydrolase 1; ENTPD1; FLJ40921; FLJ40959; Lymphoid cell activation antigen; NTPD1; NTPDase 1; NTPDase-1