• Species Reactivity

  Mouse

• Specificity

  Detects mouse IL-17 in ELISAs and Western blots. In direct ELISAs, 100% reactivity with recombinant mouse (rm) IL-17A/IL-17F heterodimer, 50% cross-reactivity with recombinant human IL-17, and 25% cross-reactivity with recombinant canine IL-17 is observed. No cross-reactivity with rmIL-17B, rmIL-17C, rmIL-17D, rmIL-17E, or rmIL-17F is observed. In Western blots, cross-reactivity with recombinant rat IL-17A is observed.    
   

• Source

  Monoclonal Rat IgG    _2A Clone # 50101

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant mouse IL‑17    
  Thr22-Ala158    
  Accession # Q62386

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  See below

• 
  

• Mouse IL-17 Sandwich Immunoassay

  Reagent    

• ELISA Capture (Matched Antibody Pair)

  2-8 µg/mL 

  Mouse IL‑17/IL‑17A Antibody (Catalog # )

• ELISA Detection (Matched Antibody Pair)

  0.1-0.4 µg/mL 

  Mouse IL‑17 Biotinylated Antibody (Catalog # )

• ELISA Standard

   

  Recombinant Mouse IL-17A Protein (Catalog # )

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17/IL-17A

Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpes virus Saimiri. cDNA clones encoding IL-17 have been isolated from activated rat, mouse and human T cells. Mouse IL-17 cDNA encodes a 158 amino acid (aa) residue precursor protein with a 21 amino acid residue signal peptide that is cleaved to yield the 137 aa residue mature  IL-17. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. At the amino acid level, mouse IL-17 shows 57% and 87% sequence identity with herpes virus and rat IL-17, respectively. An IL-17 specific mouse cell surface receptor (IL-17 R) has been cloned. While the expression of IL-17 mRNA is restricted to activated alpha beta TCR⁺CD4^-CD8^- T cells, the expression of mouse IL-17 R mRNA has been detected in virtually all cells and tissues tested. IL-17 exhibits multiple biological activities on a variety of cells including: the induction of IL-6 and IL-8 production in fibroblasts; the enhancement of surface expression of ICAM-1 in fibroblasts; activation of NF-kappa B and costimulation of T cell proliferation.

• References:

1. Kennedy, J. et al. (1996) J. Interferon Cytokine Res. 16:611.

2. Yao, Z. et al. (1995) J. Immunol. 155:5483.

3. Yao, Z. et al. (1995) Immunity 3:811.

4. Rouvier, E. et al. (1993) J. Immunol. 150:5445.

• Long Name:

  Interleukin 17

• Entrez Gene IDs:

  3605 (Human); 16171 (Mouse); 301289 (Rat); 481837 (Canine)

• Alternate Names:

  CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A