• Species Reactivity

  Human

• Specificity

  Detects human CD164 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse CD164 is observed.

• Source

  Monoclonal Mouse IgG3 Clone # 502021

• Immunogen

  NS0 mouse myeloma cell line transfected with human CD14    
  Asp24-Leu197    
  Accession # Q04900

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 750

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  PC‑3 human prostate cancer cell line

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CD164

CD164, also known as endolyn, MGC-24, and MUC-24, is an 80‑100 kDa transmembrane sialomucin protein that is expressed by epithelial cells, T and B cells, monocytes, hematopoietic progenitor cells, and activated basophils. CD164 functions as an adhesion molecule and a negative regulator of cell proliferation. A soluble isoform is generated by alternate splicing. CD164 was identified as a potential regulator of homing in a prostate cancer screening for CXCL12-responsive adhesion molecules (1). Within the ECD, human CD164 shares approximately 53% sequence identity with mouse and rat CD164.

• References:

1. Havens, A.M. et al. (2006) BMC Cancer 2006, 6:195.

• Entrez Gene IDs:

  8763 (Human); 53599 (Mouse)

• Alternate Names:

  CD164 antigen; CD164 molecule, sialomucin; CD164; Endolyn; MGC-24; MGC-24v; MUC-24; Multi-glycosylated core protein 24; sialomucin