• Species Reactivity

  Human

• Specificity

  Detects human Integrin alpha 5/CD49e.

• Source

  Monoclonal Mouse IgG1 Clone # 238307

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human Integrin alpha 5/CD49e    
  Phe42-Tyr995    
  Accession # P08648

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 750

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  K562 human chronic myelogenous leukemia cell line

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Integrin alpha 5/CD49e

The Integrin alpha 5 subunit, also called CD49e, associates with the Integrin beta 1 subunit (CD29) to form the VLA-5 complex. It functions as a receptor for fibronectin and is expressed on fibroblasts, endothelial cells, epithelial cells, platelets and some melanoma cell lines (1, 2).

• References:

1. Springer, T.A. et al. (1982) Immunol. Rev. 68:171.

2. Te Velde, A. et al. (1988) J. Immunol. 140:1548.

• Entrez Gene IDs:

  3678 (Human); 16402 (Mouse)

• Alternate Names:

  CD49 antigen-like family member E; CD49e antigen; CD49e; Fibronectin receptor subunit alpha; fibronectin receptor, alpha subunit; FNRAVLA5A; Integrin alpha 5; integrin alpha-5; Integrin alpha-F; integrin, alpha 5 (fibronectin receptor, alpha polypeptide); ITGA5; very late activation protein 5, alpha subunit; VLA-5 alpha; VLA-5