• Species Reactivity

  Mouse

• Specificity

  Detects mouse CD48/SLAMF2 in direct ELISAs and Western blots.

• Source

  Monoclonal Rat IgG1 Clone # 331504

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse CD48/SLAMF2    
  Phe23-Arg216    
  Accession # BAE96326

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 594

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  Mouse splenocytes

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CD48/SLAMF2

CD48, also known as BLAST-1, BCM-1, and SLAMF2, is a 65 kDa GPI-linked protein in the CD2 family of immunoglobulin superfamily proteins (1‑3). The mouse CD48 cDNA encodes a 240 amino acid (aa) precursor that includes a 22 aa signal sequence, a 195 aa mature protein that contains one Ig-like V-type domain and one Ig-like C2-type domain, and a 23 aa C-terminal propeptide (4). A soluble form of CD48 has been detected in the serum of lymphoid leukemia and arthritis patients (5). Mouse CD48 shares 51% and 68% aa sequence identity with human and rat CD48, respectively. It shares 18%‑26% aa sequence identity with comparable regions of mouse 2B4, BLAME, CD2F-10, CD84, CD229, CRACC, NTB-A, and SLAM. CD48 is expressed on most lineage-committed hematopoietic cells but not on hematopoietic stem cells or multipotent hematopoietic progenitors (4, 6). Among dendritic cells (DC), CD48 is selectively expressed on circulating myeloid DC and resident bone marrow and thymus DC (7). CD2, 2B4, and heparan sulfate function as CD48 ligands (8‑10). CD48 is competent to transduce signals and can also trigger signaling through CD2 or 2B4 (8, 11). CD48-CD2 interactions promote T cell activation and class switching to IgG_2a in B cells (8, 12). High affinity CD48-2B4 interactions can either promote or inhibit NK cell and cytotoxic T cell (CTL) activation (7, 11, 13, 14). CD48-2B4 ligation does not directly trigger CTL activity but enhances signaling from the T cell receptor (13). CD48-2B4 mediated inhibition of NK cell activity is distinct from MHC I-restricted mechanisms (15). CD48 expressed on NK cells is coactivating, whereas CD48 expressed on other cell types inhibits NK cell activation (14). Both CD48 expressing and nonexpressing cells can be targets of NK cell or CTL-mediated lysis (13, 16).

• References:

1. Assarsson, E. et al. (2005) J. Immunol. 175:2045.

2. Bhat, R. et al. (2006) J. Leukoc. Biol. 79:417.

3. Loertscher, R. and P. Lavery (2002) Transpl. Immunol. 9:93.

4. Wong, Y.W. et al. (1990) J. Exp. Med. 171:2115.

5. Smith, G.M. et al. (1997) J. Clin. Immunol. 17:502.

6. Keil, M.J. et al. (2005) Cell 121:1109.

7. Morandi, B. et al. (2005) J. Immunol. 175:3690.

8. Kato, K. et al. (1992) J. Exp. Med. 176:1241.

9. Latchman, Y. et al. (1998) J. Immunol. 161:5809.

10. Ianelli, C.J. et al. (1998) J. Biol. Chem. 273:23367.

11. Messmer, B. et al. (2006) J. Immunol. 176:4646.

12. Gao, N. et al. (2005) J. Immunol. 174:4113.

13. Lee, K.-M. et al. (2003) J. Immunol. 170:4881.

14. Lee, K.-M. et al. (2006) Blood 107:3181.

15. McNerney, M.E. et al. (2005) Blood 106:1337.

16. Lee, K.-M. et al. (2004) J. Exp. Med. 199:1245.

• Long Name:

  SLAM Family Member 2

• Entrez Gene IDs:

  962 (Human); 12506 (Mouse); 245962 (Rat)

• Alternate Names:

  BCM1 surface antigen; BCM1; BCM1Leukocyte antigen MEM-102; BLAST; BLAST1; BLAST1TCT.1; B-lymphocyte activation marker BLAST-1; CD48 antigen (B-cell membrane protein); CD48 antigen; CD48 molecule; CD48; hCD48; mCD48; MEM-102; SLAMF2