• Species Reactivity

  Human

• Specificity

  Detects human Syntaxin 1A in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human (rh) Epimorphin/Syntaxin 2 is observed, 25% cross-reactivity with rhSyntaxin 1B2 and rhSyntaxin 3 is observed, and no cross-reactivity with rhSyntaxin 1B1 is observed.

• Source

  Monoclonal Mouse IgG    _2A Clone # 761811

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human Syntaxin 1A    
  Met1-Leu165    
  Accession # Q16623

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  0.1 µg/mL

  See below

• 
  

• Immunohistochemistry

  8-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Syntaxin 1A

STX1A (Syntaxin [Greek for 'organizing']1A; also HPC-1) is a 34-36 kDa member of the syntaxin family of proteins. It is a t-SNARE that is widely expressed in neurons, and is involved in the exocytosis of neurotransmitters at the presynaptic membrane. STX1A is transported intracellularly by microtubule-associated syntabulin, and its availability appears to be regulated through binding to LGI3. When released from LGI3, STX1A interacts with SNAP25 and VAMP2 to form the SNARE complex involved in exocytotic vesicle release. Human STX1A is a type IV single-pass transmembrane protein (very long cytoplasmic N-terminus) that is 288 amino acids (aa) in length. It contains a 265 aa N-terminal cytoplasmic domain that contains one coiled‑coil region (aa 68-109), a t-SNARE domain with a coiled‑coil region (aa 192-254), and a C-terminal transmembrane sequence (aa 266-286). There are three potential isoform variants. One shows a 34 aa substitution for aa 227‑288, while another termed STX1C is likely soluble, and contains a 25 aa substitution for the same aa sequence above encompassing aa 227‑288. A third variant shows a five aa substitution for aa 1-10. Over aa 1-165, human STX1A shares 99% aa sequence identity with mouse STX1A.

• Entrez Gene IDs:

  6804 (Human); 20907 (Mouse); 116470 (Rat)

• Alternate Names:

  Brain Syntaxin; HPC-1; Neuron-specific antigen HPC-1; p35-1; STX1A; STX1P35-1; SYN1A; syntaxin 1A (brain); Syntaxin 1A; syntaxin-1A