• Species Reactivity

  Human

• Specificity

  Detects human Tumstatin in direct ELISAs. In direct ELISAs, less than 25% cross-reactivity with recombinant human Collagen IV alpha 1 is observed.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 762102

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human Tumstatin

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  2 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tumstatin

Tumstatin is a naturally occurring, anti-angiogenic, anti-tumor peptide of 28 kDa that constitutes the NC1 (non-collagenous) domain of the Collagen IV a3 chain [amino acids (aa) 1427-1670 of Coll IV a3;]. It is liberated from basement membranes by MMP-9, and interacts with Integrin aVb3 on endothelial cells, causing cell cycle arrest, or apoptosis of cells that are proliferating. Human Tumstatin is also active on mouse and bovine endothelial cells. Human Tumstatin shares 91% and 89% aa sequence identity with mouse and rat Tumstatin, respectively.

• Entrez Gene IDs:

  1285 (Human); 12828 (Mouse)

• Alternate Names:

  COL4A3; collagen alpha-3(IV) chain; collagen IV, alpha-3 polypeptide; collagen, type IV, alpha 3 (Goodpasture antigen); Goodpasture antigen; Tumstatin