• Species Reactivity

  Human

• Specificity

  Detects human CELSR3 in ELISA.

• Source

  Monoclonal Mouse IgG    _2B Clone # 763103

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human CELSR3    
  Val531-Ser711    
  Accession # Q9NYQ7

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  0.1 µg/mL

  See below

• 
  

• Flow Cytometry

  2.5 µg/10    ⁶ cells

  See below

• 
  

• CyTOF-ready

  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CELSR3

CELSR3 (Cadherin EGF LAG Seven-pass G-type Receptor 3; also cadherin family member 11/CDHF11, FMI1 and EGFL1) is a 355 kDa (predicted) member of the LN-7TM subfamily, GPCR 2 family of proteins. It is coexpressed with Fzd3 on postmigratory neurons of the developing DRG and cranial ganglia, and appears to serve as an axonal guidance cue. Mature human CELSR3 is 3280 amino acids (aa) in length (aa 33-3312). It is a highly complex 7-transmembrane protein that contains a 2508 aa extended N-terminal extracellular region (aa 33-2540) plus a 538 aa C-terminal cytoplasmic domain. The N-terminal region contains nine consecutive cadherin domains (aa 326-1265), followed by a mixture of eight EGF-like and three laminin-like domains, and one membrane-proximal GPS domain. There are two potential isoform variants. One shows a five aa insertion after Gly2158, while another possesses an alternative start site at Met276. Over aa 531-711, human CELSR3 shares 98% aa sequence identity with mouse CELSR3.

• Long Name:

  Cadherin, EGF LAG Seven-pass G-type Receptor 3

• Entrez Gene IDs:

  1951 (Human); 107934 (Mouse); 83466 (Rat)

• Alternate Names:

  Cadherin family member 11; cadherin, EGF LAG seven-pass G-type receptor 3 (flamingo homolog, Drosophila); CDHF11; CDHF11RESDA1; CELSR3; EGFL1; EGFL1cadherin EGF LAG seven-pass G-type receptor 3; EGF-like protein 1; EGF-like-domain, multiple 1; epidermal growth factor-like 1; Epidermal growth factor-like protein 1; Flamingo homolog 1; FMI1anchor protein; HFMI1; KIAA0812; MEGF2; MEGF2cadherin EGF LAG seven-pass G-type receptor 3, flamingo (Drosophila) homolog; multiple EGF-like domains 2; Multiple EGF-like domains protein 2; Multiple epidermal growth factor-like domains protein 2; RESDA1