• Species Reactivity

  Human

• Specificity

  Detects human BetaTrCP1 isoform 2 in direct ELISAs and Western blots. Detects mouse BetaTrCP1 in Western Blots. Detection of mouse BetaTrCP1 in Immuncytochemistry has not been tested.    
   

• Source

  Monoclonal Mouse IgG    ₁ Clone # 763524

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human beta -TrCP1 isoform 2    
  Met1-Leu120    
  Accession # Q9Y297-2

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  See below

• 
  

• Simple Western

  10 µg/mL

  See below

• 
  

• Immunocytochemistry

  8-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: beta-TrCP1/BTRC

beta -TrCP1, also called BTRC ( beta -transducin repeat containing E3 ubiquitin ligase), FBW1A (F-box/WD repeat containing 1A) and FBXW1A, is an approximately 63-70 kDa protein that associates with Skp1, Cullin, and RBX1 to form an SCF ubiquitin ligase complex. These complexes regulate the degradation of a wide variety of proteins including transcription factors, signal transduction molecules, and cytokine receptors. They play important roles in regulation of the inflammatory response, cell cycle, apoptosis, tumorigenesis, and the response to DNA damage. beta -TrCP1 can form homodimers and heterodimers with beta -TrCP2, which associate into SCF complexes with distinct substrate preferences. Human beta -TrCP1 contains a dimerization domain (aa 128-177), an F-box domain (aa 181-228), and seven tandem WD repeats (aa 301‑590). The WD repeats assemble into a beta propeller structure that binds to phosphorylated destruction motifs in the substrate. Alternate splicing generates a short isoform with a 36 aa deletion near the N-terminus. Within aa 1-120 of the 63 kDa short isoform, human beta -TrCP1 shares 98% and 91% aa sequence identity with mouse and rat beta -TrCP1, respectively.

• Long Name:

  Beta-Transducin Repeat Containing E3 Ubiquitin Protein Ligase

• Entrez Gene IDs:

  8945 (Human); 12234 (Mouse); 361765 (Rat)

• Alternate Names:

  beta-transducin repeat containing; betaTrCP; BETA-TRCP; betaTrCP1; beta-TrCP1; BTRC; BTRCP; bTrCP1; E3RSIkappaB; F-box and WD repeats protein beta-TrCP; F-box/WD repeat-containing protein 1A; FBW1A; FBXW1; FBXW1A; FBXW1ABTRCP; FWD1; MGC4643; pIkappaBalpha-E3 receptor subunit