• Species Reactivity

  Mouse

• Specificity

  Detects mouse Galectin-9 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Galectin-9 or recombinant mouse Galectin-4 is observed.

• Source

  Monoclonal Rat IgG    _2B Clone # 766428

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant mouse Galectin-9 (short isoform)    
  Ala2-Thr322    
  Accession # O08573-2

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Immunohistochemistry

  8-25 µg/mL

  See below

• 
  

• CyTOF-ready

  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

• 
  

• Intracellular Staining by Flow Cytometry

  2.5 µg/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Galectin-9

Mouse Galectin-9 ( beta -galactoside-binding lectin 9) is a 36-39 kDa, secreted, S-type lectin. It is 353 amino acids in length and contains no identifiable signal sequence. There are two distinct carbohydrate-binding regions (aa 81-87 and 285-291) that are joined by a linker region (aa 148-205). At least one alternate splice form exists that shows a 31 aa insertion between aa 147-148. The short form is expressed on fibroblasts, hepatocytes, endothelial cells and astrocytes. The long form is expressed on intestinal epithelium. Galectin-9 binds TIM-3 on Th1 cells, inducing apoptosis. Mouse Galectin-9 is 69% and 85% aa identical to human and rat Galectin-9, respectively.

• Entrez Gene IDs:

  3965 (Human); 16859 (Mouse)

• Alternate Names:

  Ecalectin; GAL9; gal-9; galectin 9; Galectin9; Galectin-9; HUAT; lectin, galactoside-binding, soluble, 9; LGALS9; LGALS9A; MGC117375; MGC125973; MGC125974; Tumor antigen HOM-HD-21; urate transporter/channel protein