• Species Reactivity

  Human

• Specificity

  Detects human DISP1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) DISP2 or rhSMAGP is observed.

• Source

  Monoclonal Mouse IgG    _2B Clone # 768213

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human DISP1    
  Glu212-Lys492 and Ser746-Thr984    
  Accession # Q96F81

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Immunocytochemistry

  8-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DISP1

DISP1 is a multispan (12) transmembrane protein with cytoplasmic N- and C-terminal tails. It contains a stero/sensing domain (SSD) and has been shown to play a role in regulating the release of cholesterol-modified SHH. Within the regions used for immunization, human and mouse DISP1 share 93% amino acid sequence homology.

• Long Name:

  Dispatched 1

• Entrez Gene IDs:

  84976 (Human); 68897 (Mouse); 289338 (Rat)

• Alternate Names:

  DISP1; DISPA; DISPAFLJ43740; dispatched A; dispatched homolog 1 (Drosophila); Dispatched; DKFZp434I0428; MGC104180; MGC13130; MGC16796; protein dispatched homolog 1