• Species Reactivity

  Human

• Specificity

  Detects human ABCA1 in direct ELISAs.

• Source

  Monoclonal Mouse IgG    _2A Clone # 768510

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human ABCA1    
  Phe1248-Gln1350    
  Accession # O95477

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• CyTOF-ready

  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

• 
  

• Intracellular Staining by Flow Cytometry

  0.25 µg/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ABCA1

ABCA1 (ATP-binding cassette, subfamily A member 1; also cholesterol efflux regulatory protein) is a 240-280 kDa member of the ABCA family, ABC transporter superfamily of molecules. It is widely expressed, particularly by macrophages, where it participates in the transport of lipid across cell membranes. Once transported, it is passed on to tethered apoA-I to form HDL. Human ABCA1 is 2261 amino acids (aa) in length. It has up to 15 transmembrane segments with beginning and ending cytoplasmic domains and a large cytoplasmic central region. ABC transporters are found between aa 899-1311 and 1912-2144. Amino acids 2216-2221 are essential for lipid transport, and two intramolecular disulfide bonds are required for activity (Cys75-Cys309 and Cys1463/1465-Cys1477). There are three potential isoform variants. One possesses an alternative start site at Met61, while two others contain 12 aa and 123 aa substitutions for aa 241-2261, respectively. Over aa 1248-1350, human ABCA1 shares 97% aa identity with mouse ABCA1.

• Long Name:

  ATP-binding Cassette, Sub-family A (ABC1), Member 1

• Entrez Gene IDs:

  19 (Human); 11303 (Mouse)

• Alternate Names:

  ABC1; ABC-1; ABC1ATP-binding cassette transporter A1; ABCA1; ATP-binding cassette 1; ATP-binding cassette transporter 1; ATP-binding cassette, sub-family A (ABC1), member 1; CERP; Cholesterol efflux regulatory protein; EC 2.7.7.8; EC 2.8.1.8; EC 3.6.3; EC 3.6.3.41; FLJ14958; HDLDT1; membrane-bound; MGC164864; MGC165011; TGD; TGDATP-binding cassette sub-family A member 1