• Species Reactivity

  Human

• Specificity

  Detects human Spi-B in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Spi-B is observed.

• Source

  Monoclonal Mouse IgG    _2B Clone # 769653

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human Spi-B    
  Ala7-Leu115    
  Accession # Q01892

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Spi-B

Spi-B is a 33-45 kDa member of the ets family of transcription factors. It is found in hematopoietic cells such as B cells and plasmacytoid dendritic cells (DC). In transitional B cells, Spi-B promotes their differentiation into follicular (naïve) B cells. In hematopoietic stem cells, Spi-B stimulates the generation of IFN-producing plasmacytoid DC at the expense of T, B and NK cell development. The 262 amino acid (aa) human Spi-B contains a dual transactivation region (aa 1-62) and an ets DNA-binding domain (aa 169-252). One 177 aa variant diverges after aa 162. Over aa 7-115, human Spi-B shares 72% and 70% aa identity with mouse and rat Spi-B, respectively.  

• Long Name:

  Transcription Factor Spi [Spleen FFV Proviral Integration Oncogene] B

• Entrez Gene IDs:

  6689 (Human); 272382 (Mouse); 499146 (Rat)

• Alternate Names:

  Spi-B transcription factor (Spi-1/PU.1 related); SpiB; Spi-B; transcription factor Spi-B