• Species Reactivity

  Mouse

• Specificity

  Detects mouse Kynureninase in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Kynureninase is observed.

• Source

  Monoclonal Rat IgG    _2B Clone # 771312

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Chinese hamster ovary cell line CHO-derived recombinant mouse Kynureninase    
  Met1-Ser464    
  Accession # Q9CXF0

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  0.5 µg/mL

  See below

• 
  

• Immunohistochemistry

  8-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kynureninase

Kyureninase (KYNU, a hydrolase that acts on dog [Greek: kyon] urine [Greek: ouron]) is a 50-55 kDa member of the kyureninase family of enzymes.  It is a pyridoxal phosphate-dependent cytosolic hydrolase that occurs in a variety of cell types, including hepatocytes and macrophages.  Kyureninase participates in the metabolism of tryptophan.  Dietary Trp is either incorporated into protein, or metabolized into niacin, serotonin or NAD.  Kyureninase catalyzes one of two steps that lead to the formation of a key downstream intermediate called 3-hydroxyanthranilic acid.  Mouse Kyureninase is 464 amino acids (aa) in length.  It contains one large catalytic site (aa 24-462), and typically functions as a noncovalent homodimer.  There are at least two potential isoform variants.  One contains a three aa substitution for aa 425-464, while another possesses a 22 aa substitution for aa 302-464.  Full-length mouse Kyureninase shares 92% and 83% aa sequence identity with rat and human Kyureninase, respectively.      

• Entrez Gene IDs:

  8942 (Human); 70789 (Mouse); 116682 (Rat)

• Alternate Names:

  EC 3.7.1.3; KYNU; kynureninase (L-kynurenine hydrolase); Kynureninase; L-kynurenine hydrolase