• Species Reactivity

  Mouse

• Specificity

  Detects mouse Metadherin in direct ELISAs.    
   

• Source

  Monoclonal Rat IgG    _2B Clone # 754523

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant mouse Metadherin    
  Lys167-Ser297    
  Accession # Q80WJ7

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Metadherin

Metadherin (MTDH; also Lyric and Astrocyte-elevated gene-1/AEG1) is a unique molecule originally discovered to be upregulated in astrocytes following HIV infection. Although its predicted MW is 64 kDa, it runs anomalously at approximately 80 kDa in SDS-Page. It is widely expressed, and appears to be a component of the ER, nucleolus and inner nuclear membrane. Metadherin is reported to promote Akt/PI3 kinase activity, and suppress apoptosis-associated FOXO3A transcription. Mouse Metadherin is a type III (i.e.- a type I with no signal sequence) transmembrane protein 579 amino acids (aa) in length. It contains a luminal N-terminus (aa 1-48) with a 510 aa cytoplasmic C-terminus. There are no identifiable structural motifs, although two poly-Lys segments and two utilized Ser phosphorylation sites exist. Multiple bands at 80 kDa, 75 kDa, 50-55 kDa and 37 kDa are seen for Metadherin on SDS-Page. They may reflect the presence of at least two potential isoform variants that show 1) a deletion of aa 422-457, and 2) an Ile substitution for aa 190-579. Over aa 167-297, mouse Metadherin shares 98% and 97% aa sequence identity with rat and human Metadherin, respectively.

• Entrez Gene IDs:

  92140 (Human); 67154 (Mouse); 170910 (Rat)

• Alternate Names:

  3D3; AEG1; AEG-13D3/LYRIC; AEG1LYRIC/3D3; Astrocyte elevated gene-1 protein; LYRIC; LYRICMetastasis adhesion protein; Lysine-rich CEACAM1 co-isolated protein; Metadherin; MTDH; protein LYRIC