• Species Reactivity

  Human

• Specificity

  Detects human NGL-1/LRRC4C in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) LRRC4 or rhNGL‑3/LRRC4B is observed.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 755720

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human NGL-1/LRRC4C    
  Gln45-Lys527 (predicted)    
  Accession # Q9HCJ2

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Immunohistochemistry

  8-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NGL-1/LRRC4C

Human NGL-1 (Netrin-G1 ligand; gene name LRRC4C) is a 67 kDa (predicted for mature protein), type I transmembrane cell adhesion molecule that is a member of the NGL family of proteins (1, 2). It is synthesized from a precursor that is 640 amino acids (aa) in length that contains a 44 aa signal sequence, a 483 aa extracellular region, a 21 aa transmembrane region, and a short cytoplasmic tail of 92 aa. The extracellular region of NGL-1 consists of nine leucine-rich repeats (LRRs) that are flanked by LRR N-terminal and LRR C-terminal domains, and followed by an Ig-like C2‑type domain (1, 2). The cytoplasmic region contains a C‑terminal Glu-Thr-Gln-Ile sequence that corresponds to a potential PDZ (postsynaptic density-95/discs large/zona occludens-1) domain-binding motif (1, 2). Human NGL-1 shares 99.7% aa sequence identity with mouse NGL-1. Mouse NGL-1 is highly expressed in the developing cerebral cortex and the striatum at embryonic day 14 (1). Postnatally, NGL-1 is expressed exclusively in the brain, with the highest expression found in the cerebral cortex as a whole, and in individual neocortical areas such as the frontal, parietal and occipital lobes (1). Moderate expression of NGL-1 occurs in the putamen, amygdala, hippocampus and medulla oblongata (1). Weak expression is found in the caudate nucleus and thalamus (1). Functionally, membrane-bound cell-surface NGL-1 binds to netrin-G1 specifically through its LRR region, and in the developing brain, may promote neurite outgrowth of thalamocortical axons (1‑4).

• References:

1. Lin, J.C. et al. (2003) Nat. Neurosci. 6:1270.

2. Kim, S. et al. (2006) Nat. Neurosci. 9:1294.

3. Chen, Y. et al. (2006) Brain Res. Rev. 51:265.

4. Nishimura-Akiyoshi, S. et al. (2007) Proc. Natl. Acad. Sci. U.S.A. 104:14801.

• Long Name:

  Netrin-G1 Ligand/Leucine-rich Repeat Containing 4C

• Entrez Gene IDs:

  57689 (Human); 241568 (Mouse); 311236 (Rat)

• Alternate Names:

  KIAA1580NGL-1NGL1Netrin-G1 ligand; leucine rich repeat containing 4C; leucine-rich repeat-containing protein 4C; LRRC4C; NGL1; NGL-1