• Species Reactivity

  Human

• Specificity

  Detects human DPP9 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) DPPIV/CD26 or rhDPP10 is observed.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 757004

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  S. frugiperda insect ovarian cell line     Sf 21-derived recombinant human DPP9    
  Arg2-Leu892 (predicted)    
  Accession # Q1ZZB8

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  See below

• 
  

• Simple Western

  10 µg/mL

  See below

• 
  

• Immunocytochemistry

  8-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DPP9

DPP9 is a member of the S9b family of serine peptidases (1, 2). It shares 19% amino acid identity with DPP4 and 58% amino acid identity with DPP8. It exhibits post‑proline dipeptidyl aminopeptidase activity, cleaving Xaa-Pro dipeptides from the N-terminus of oligo- and polypeptides (3). Unlike DPP4, DPP9 does not appear to be membrane bound and is localized exclusively in the cytoplasm (4). This family of proline-specific dipeptidyl peptidases has been implicated in a variety of diseases including type 2 diabetes, obesity and cancer, and has been a potential target for drug discovery (5, 6).

• References:

1. Olsen, C. and Wagtmann, N. (2002) Gene 299:185.

2. Qi, S.Y. et al. (2003) Biochem. J. 373:179.

3. Bjelke, J.R. et al. (2006) Biochem. J. 396:391.

4. Ajami, K. et al. (2004) Biochim. Biophys. Acta. 1679:18.

5. Rosenblum, J.S. and Kozarich, J.W. et al. (2003) Curr. Opin. Chem. Biol. 7:496.

6. Van der Veken, P. et al. (2007) Curr. Top. Med. Chem. 7:621.

• Long Name:

  Dipeptidyl peptidase 9

• Entrez Gene IDs:

  91039 (Human); 224897 (Mouse); 301130 (Rat)

• Alternate Names:

  dipeptidyl peptidase 9; Dipeptidyl peptidase IV-related protein 2; dipeptidyl peptidase IV-related protein-2; Dipeptidyl peptidase IX; Dipeptidyl peptidase-like protein 9; dipeptidylpeptidase 9; dipeptidyl-peptidase 9; DKFZp762F117; DP9; DPLP9; DPP IX; DPP9; DPRP2; DPRP-2; EC 3.4.14.5; FLJ16073