• Species Reactivity

  Human

• Specificity

  Detects human Semaphorin 3C in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant mouse Semaphorin 3C and no cross-reactivity with recombinant human Semaphorin 3A, 3B, 3D, or 3F is observed.

• Source

  Monoclonal Mouse IgG    _2B Clone # 757820

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human Semaphorin 3C    
  Gly21-Ser738    
  Accession # Q99985

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Immunocytochemistry

  8-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Semaphorin 3C

Semaphorin 3C (Sema3C; previously semaE) is one of six Class 3 secreted semaphorins which share 40-50% amino acid (aa) identity. Class 3 semaphorins are potent chemorepellents that function in axon and/or vascular guidance during development, and may be upregulated in tumor progression (1, 2). The 751 amino acid (aa) mouse Sema3C is highly modular. It contains a 20 aa signal sequence, an ~500 aa N-terminal Sema domain that forms a beta -propeller structure similar to that found in integrin molecules, a cysteine knot, a furin-type cleavage site, an Ig-like domain, and a C-terminal basic domain (1-3). Covalent dimerization plus cleavage at the C-terminus are required for activity of class 3 semaphorins (4). Mouse Sema3C shares at least 95% aa identity with human, rat, cow and dog Sema3C, and 89% and 75% aa identity with chick and zebrafish Sema3C, respectively. Type 3 semaphorins transduce signals through transmembrane plexins, either directly or by binding associated neuropilin receptors (1, 2). Sema3C signaling is transduced by Plexin-D1 indirectly via Neuropilin-1 or Neuropilin-2 receptors (5). Sema3C is expressed in all somitic motor neurons, in lung buds and in cardiac neural crest cells during development (1, 5-8). Sema3C activates integrins in certain cells so, in addition to its repulsive activities, it sometimes acts as a chemoattractant (6, 9). In the developing nervous system, this chemoattraction appears to complement Sema3A repulsion in adjacent cell layers (1, 6, 7). Sema3C also provides an attractive force opposing Sema6A and Sema6B to guide migration of neural crest endothelial cells to the cardiac outflow tract (10). Consequently, defects in aortic arch formation occur when Sema3C or Plexin-D1 genes or Sema3C-neuropilin interactions are disrupted (5, 11, 12).

• References:

1. Hinck, L. (2004) Dev. Cell 7:783.

2. Neufeld, G. et al. (2005) Front. Biosci. 10:751.

3. Gherardi, E. et al. (2004) Curr. Opin. Struct. Biol. 14:669.

4. Adams, R. H. et al. (1997) EMBO J. 16:6077.

5. Gitler, A. D. et al. (2004) Dev. Cell 7:107.

6. Bagnard, D. et al. (1998) Development 125:5043.

7. Cohen, S. et al. (2005) Eur. J. Neurosci. 21:1767.

8. Puschel, A. W. et al. (1995) Neuron 14:941.

9. Herman, J. G. and G. G. Meadows (2007) Int. J. Oncol. 30:1231.

10. Toyofuku, T. et al. (2008) Dev. Biol. 321:251.

11. Feiner, L. et al. (2001) Development 128:3061.

12. Gu, C. et al. (2003) Dev. Cell 5:45.

• Entrez Gene IDs:

  10512 (Human); 20348 (Mouse)

• Alternate Names:

  (semaphorin) 3C; sema domain, immunoglobulin domain (Ig), short basic domain, secreted; Sema E; SEMA3C; SEMAE; Semaphorin 3C; Semaphorin E; semaphorin-3C; semaphorin-E; SEME