• Species Reactivity

  Human

• Specificity

  Detects human LRRC3B in direct ELISAs and Western blots.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 758315

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Chinese hamster ovary cell line CHO-derived recombinant human LRRC3B    
  Phe28-Ala205    
  Accession # Q96PB8

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  2 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LRRC3B

LRRC3B, also known as LRP15, is a transmembrane leucine-rich protein that is downregulated by promoter methylation in gastric and colorectal cancers. It confers resistance to ultraviolet light-induced DNA damage and functions as a tumor suppressor. Mature human LRRC3B contains an LRR N-terminal domain (aa 34-64), 3 LRR repeats (aa 65-135), an LRR C-terminal domain (aa 145-197), a transmembrane segment (aa 205-225), and a C-terminal region (aa 226-259). Within aa 28-205, human LRRC3B shares 100% aa sequence identity with mouse and rat LRRC3B.

• Long Name:

  Leucine Rich Repeat Containing 3B

• Entrez Gene IDs:

  116135 (Human); 218763 (Mouse)

• Alternate Names:

  leucine rich repeat containing 3B; leucine-rich repeat-containing protein 3B; LRP15; LRP15Leucine-rich repeat protein LRP15; LRRC3B; MGC102927