• Species Reactivity

  Mouse

• Specificity

  Detects mouse IL-36Ra/IL-1F5 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant human IL-36Ra and recombinant mouse IL-1ra is observed.

• Source

  Monoclonal Rat IgG    _2B Clone # 759207

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant mouse IL-36Ra/IL-1F5    
  Met1-Cys155    
  Accession # Q9QYY1

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-36Ra/IL-1F5

Mouse interleukin-36 receptor antagonist (IL-36Ra; previously IL-1F5; also named FIL-1δ [delta], IL‑1HY1, IL-1H3, and IL-1L1) is a member of the IL‑1 family of proteins (1-6). IL‑1 family members include IL-1 beta, IL-1 alpha, IL-1ra, IL‑18 and IL‑1F5 through F10 (6, 7). All family members show a 12 beta -strand, beta -trefoil configuration, and are believed to have arisen from a common ancestral gene that underwent multiple duplications (8). The mouse IL‑36Ra/IL‑1F5 gene maps to a region on mouse chromosome 2 that contains all other IL-1 family members (except IL‑18), supporting an evolutionary relationship with the IL-1 family (1, 9). It is particularly close to the gene for IL-1ra and is likely a relatively recent duplication of that gene. IL‑36Ra/IL‑1F5 is synthesized as a 156 amino acid (aa) protein that contains no signal sequence, no prosegment and no potential N-linked glycosylation site(s) (2, 5, 8). Nevertheless, it appears to be secreted as a 17 kDa monomer. In humans, there is an alternate start site that potentially gives rise to an alternate splice form (5). This translated product has a premature stop codon, resulting in a truncated 16 aa peptide. Mouse to human, full length IL‑36Ra/IL‑1F5 has 90% aa identity. Within the family, IL-36Ra/IL-1F5 is 48%, 30%, 35%, 35%, 35%, 37% and 43% aa identical to IL‑1ra, IL‑1 beta, IL‑36 alpha /IL‑1F6, IL‑37/IL‑1F7, IL-36 beta /IL-1F8, IL‑36 gamma /IL‑1F9 and IL‑1F10, respectively. Cells reported to express IL-36Ra/IL-1F5 include monocytes, B cells, dendritic cells/Langerhans cells, keratinocytes, and gastric fundus Parietal and Chief cells (1, 8). Indirect evidence suggests the receptor for IL-36Ra/IL-1F5 is IL‑1 Rrp2 and/or IL-1 RAcP (9). IL-36Ra/IL-1F5 was initially reported to be an antagonist of  IL-36 gamma activity (4, 7). This would be consistent with its hypothesized relationship to IL‑1ra.

• References:

1. Smith, D. E. et al. (2000) J. Biol. Chem. 275:1169.

2. Kumar, S. et al. (2000) J. Biol. Chem. 275:10308.

3. Mulero, J.J. et al. (1999) Biochem. Biophys. Res. Commun. 263:702.

4. Nicklin, M.J.H. et al. (2002) Genomics. 79:718.

5. Barton J. L. et al. (2000) Eur. J. Immunol. 30:3299.

6. Dinarello, C. et al. (2010) Nat. Immunol. 11:973.

7. Dunn, E. et al. (2001) Trends Immunol. 22:533.

8. Debets, R. et al. (2001) J. Immunol. 167:1440.

9. Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.

• Long Name:

  Interleukin 36 Receptor Antagonist/Interleukin 1 Family 5

• Entrez Gene IDs:

  26525 (Human); 54450 (Mouse)

• Alternate Names:

  FIL1 delta; IL1F5; IL-1HY1; IL-1L1; IL-1RP3; IL36Ra; IL-36Ra; IL36RN; Interleukin 36 Receptor Antagonist; Interleukin-36 Receptor Antagonist