• Purity

  >95%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.01 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR4.

  The ED₅₀ for this effect is 4-24 ng/mL.

• Source

  E. coli-derived Gly21-Asn119

• Accession #

• N-terminal Sequence    
  Analysis

  Gly21, Lys22 & Pro23    
   

• Predicted Molecular Mass

  11.6 kDa

• SDS-PAGE

  14 kDa, reducing conditions

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: CXCL12/SDF-1 gamma

Human CXCL12 is expressed as five isoforms that differ only in the C-terminal tail (1). The gamma  isoform of CXCL12, also known as SDF-1 gamma, is a 12 kDa, heparin-binding member of the CXC (or alpha) family of chemokines (2, 3). Mature SDF-1 molecules are not glycosylated and exhibit a typical three antiparallel beta -strand chemokine-like fold. N-terminal aa 1 ‑ 8 form a receptor binding site, while aa 1 and 2 (Lys‑Pro) are involved in receptor activation (4). All SDF-1 isoforms can undergo proteolytic processing of the first two N-terminal amino acids by CD26, which is thought to create a reduced‑activity chemokine (5). Human SDF-1 gamma is synthesized as a 119 amino acid (aa) precursor that contains a 21 aa signal sequence and a 98 aa mature region (1). Mature human SDF-1 gamma shares 99%, 97% and 98% aa identity with mouse, rat, and equine SDF-1 gamma, respectively. The unique C‑terminal 26 aa of SDF-1 gamma  are highly charged, including four BBXB (where B = basic and X = any aa) motifs, while the most prevalent form, SDF-1 alpha, has 4 unique C‑terminal aa and binds heparin via the shared BBXB site more N‑terminally located (2, 6). The SDF‑1 gamma C‑terminus binds heparin in secreted SDF-1 gamma, or targets the isoform to the nucleolus in the absence of a signal sequence (6 ‑ 8). SDF-1 isoforms interact with CXCR4 and CXCR7 receptors on the cell surface, and can also bind syndecan-4 (9 ‑ 12). SDF-1 alpha or beta are known to influence lymphopoiesis, enhance the survival of myeloid progenitor cells, regulate the patterning and cell number of neural progenitors, and promote angiogenesis (2). Of all SDF-1 isoforms, SDF-1 gamma is the most strongly attached to the cell surface via glycans, least likely to circulate, and most active in binding CXCR4 and blocking HIV entry to cells via CXCR4 (9, 13). Unlike other isoforms, it is constitutively expressed mainly in the heart or rodent brain, and is not expressed prenatally (14 ‑ 16).

• References:

1. Yu, L. et al. (2006) Gene 374:174.

2. Janowski, M. (2009) Cell Adh. Migr. 3:243.

3. Zlotnik, A. and O. Yoshie (2000) Immunity 12:121.

4. Crump, M.P. et al. (1997) EMBO J. 16:6996.

5. De La Luz Sierra, M. et al. (2004) Blood 103:2452.

6. Rueda, P. et al. (2007) PLoS ONE 7:e2543.

7. Laguri, C. et al. (2007) PLoS ONE 10:e1110.

8. Torres, R. and J.C. Ramirez (2009) PLoS ONE 10:e7570.

9. Altenburg, J.D. et al. (2010) J. Virol. 84:2563.

10. Balabanian, K. et al. (2005) J. Biol. Chem. 280:35760.

11. Levoye, A. et al. (2009) Blood 113:6085.

12. Charnaux, N. et al. (2005) FEBS J. 272:1937.

13. Altenburg, J.D. et al. (2007) J. Virol. 81:8140.

14. Gleichmann, M. et al. (2000) Eur. J. Neurosci. 12:1857.

15. Segret, A. et al. (2007) J. Histochem. Cytochem. 55:141.

16. Franco, D. et al. (2009) Anat. Rec. (Hoboken) 292:891.

• Long Name:

  CXCL12/Stromal cell-Derived Factor 1

• Entrez Gene IDs:

  6387 (Human)

• Alternate Names:

  CXCL12/SDF-1 gamma; SDF1 gamma; SDF1g