• Species Reactivity

  Human, Mouse, Rat

• Specificity

  Detects human Gelsolin/GSN in ELISAs. Detects human, mouse and rat Gelsolin/GSN in Western Blots.    
   

• Source

  Monoclonal Mouse IgG    ₁ Clone # 893205

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  HEK293 human embryonic kidney cell line transfected with human Gelsolin/GSN    
  Met1-Ala782    
  Accession # P06396

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  0.5 µg/mL

  See below

• 
  

• Simple Western

  5 µg/mL

  See below

• 
  

• Immunohistochemistry

  8-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Gelsolin/GSN

Gelsolin, also known as GSN, actin-depolymerizing factor/ADF, AGEL and Brevin, is a 90-95 kDa member of the villin/gelsolin family.  Widely expressed, Gelsolin binds to actin and fibronectin, and is found both secreted in plasma and in cytoplasm.  Cytoplasmic Gelsolin lacks 51 N-terminal amino acids (aa) present in the secreted protein.  Gelsolin was identified by its ability to sever actin filaments in the presence of submicromolar calcium, and plays a role in ciliogenesis.  Defects in Gelsolin cause amyloidosis type 5, a hereditary disease characterized by gelsolin amyloid deposition.  Full-length human Gelsolin is 782 aa and shares 93% aa identity with mouse and rat Gelsolin.

• Entrez Gene IDs:

  2934 (Human); 227753 (Mouse); 296654 (Rat)

• Alternate Names:

  Actin-depolymerizing factor; ADF; AGEL; Brevin; DKFZp313L0718; gelsolin (amyloidosis, Finnish type); Gelsolin; GSN