• Species Reactivity

  Human

• Specificity

  Detects HEK293 cells transfected with human NHA2/SLC9B2 by Flow Cytometry. Does not detect untransfected or irrelevant transfected HEK293 cells.

• Source

  Monoclonal Mouse IgG    _2B Clone # 896151

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  NS0 mouse myeloma cell line transfected with human NHA2/SLC9B2    
  Met1-Val537    
  Accession # NP_849155

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25 µg/10    ⁶ cells

  See below

• 
  

• CyTOF-ready

  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NHA2/SLC9B2

NHA2/SLC9B2 (Solute Carrier family 9, subfamily B, member 2) is a member of the solute carrier family of multi-pass membrane proteins. Sodium hydrogen antiporters, such as SLC9B2 (NHA2), convert the proton motive force established by the respiratory chain or the F1F0 mitochondrial ATPase into sodium gradients that drive other energy-requiring processes, transduce environmental signals into cell responses, or function in drug efflux. SLC9B2 contributes to organellar volume homeostasis and is required for osteoclast differentiation and bone resorption activity.

• Long Name:

  Sodium/Hydrogen Cation Proton Antiporter 2

• Entrez Gene IDs:

  133308 (Human); 7086 (Mouse); 679958 (Rat)

• Alternate Names:

  FLJ23984; Mitochondrial Na(+)/H(+) exchanger NHA2; mitochondrial sodium/hydrogen exchanger NHA2; Na(+)/H(+) exchanger-like domain-containing protein 2; Na+/H+ exchanger domain containing 2; NHA2; NHA2NHE10; NHE domain-containing protein 2; NHE10; NHEDC2; SLC9B2; Sodium/hydrogen exchanger-like domain-containing protein 2