• Species Reactivity

  Human

• Specificity

  Detects human L1TD1 in direct ELISAs and Western blots.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 901258

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human L1TD1    
  Met1-Leu142    
  Accession # Q5T7N2

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  See below

• 
  

• Immunocytochemistry

  8-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: L1TD1

L1TD1 (LINE-1 type transposase domain-containing protein 1; also known as ES cell-associated protein 11 and FLJ10884) is an RNA binding protein with a reported molecular weight of approximately 100 kDa.  It is 865 amino acids (aa) in length and shares 43% aa identity with mouse L1TD1.  L1TD1 is a marker for undifferentiated pluripotent stem cells.  Knock down of its expression in these cells has been shown to decrease the expression of critical pluripotency factors such as Nanog and Oct-3/4.  L1TD1 function in stem cells is likely regulated by its interaction with factors such as LIN-28 and is suggested to regulate RNA processing.

• Long Name:

  LINE-1 Type Transposase Domain Containing 1

• Entrez Gene IDs:

  54596 (Human); 381591 (Mouse); 685355 (Rat)

• Alternate Names:

  ECAT11; ES cell-associated protein 11; FLJ10884; L1TD1; LINE-1 type transposase domain containing 1; LINE-1 type transposase domain-containing protein 1; MGC133253; RP5-1155K23.3