• Species Reactivity

  Human

• Specificity

  Detects human Tiki1/TRABD2A in direct ELISAs and Western blots.

• Source

  Monoclonal Mouse IgG    _2A Clone # 901705

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Chinese hamster ovary cell line CHO-derived recombinant human Tiki1/TRABD2A    
  Met1-Met477    
  Accession # Q86V40

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  2 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tiki1/TRABD2A

Tiki1, also known as TRAB Domain-containing protein 2A (TRABD2A) and C2orf89, is an evolutionarily conserved transmembrane metalloprotease that acts as an inhibitor of the Wnt signaling pathway. The term Tiki refers to a large-headed humanoid in Polynesian mythology, and Tiki1 was initially identified by functional screening as an organizer-specific protein that is required for head formation in Xenopus. Tiki1 negatively regulates Wnt signaling by mediating the cleavage of the eight N-terminal residues from a subset of Wnt proteins, including Wnt3A and Wnt5, but not Wnt11. Following this cleavage, Wnt proteins become oxidized and form large disulfide-bond oligomers, leading to their inactivation. Human Tiki1 is 505 amino acids (aa) in length; a second shorter isoform has also been identified that is missing aa 225-273.

• Entrez Gene IDs:

  129293 (Human)

• Alternate Names:

  C2orf89; LOC129293; Tiki1; TRABD2A