详细说明
Species Reactivity
Human
Specificity
Stains human SLC5A5 transfectants but not irrelevant transfectants in flow cytometry.
Source
Monoclonal Mouse IgG 2A Clone # 902320
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human SLC5A5
Accession # Q92911Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated
Applications
Recommended
ConcentrationSample
Flow Cytometry
0.25 µg/10 6 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Flow Cytometry | Detection of SLC5A5 in HEK293 Human Cell Line Transfected with Human SLC5A5 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human SLC5A5 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human SLC5A5 Monoclonal Antibody (Catalog # MAB8367) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # ). Quadrant markers were set based on control antibody staining (Catalog # ). |
Preparation and Storage
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SLC5A5
Long Name:
Solute Carrier Family 5 (Sodium Iodide Symporter), Member 5
Entrez Gene IDs:
6528 (Human); 114479 (Mouse); 114613 (Rat)
Alternate Names:
Na(+)/I(-) cotransporter; Na(+)/I(-) symporter; NIS; NISNa(+)/I(-)-symporter; SLC5A5; sodium/iodide cotransporter; Sodium-iodide symporter; solute carrier family 5 (sodium iodide symporter), member 5; Solute carrier family 5 member 5; TDH1