• Species Reactivity

  Human

• Specificity

  Detects human Serum Amyloid A1 in direct ELISAs.

• Source

  Monoclonal Mouse IgG    ₃ Clone # 902738

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human Serum Amyloid A1    
  Accession # P0DJI8

• Formulation

  Lyophilized from a 0.2 μm filtered solution in TBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Immunohistochemistry

  8-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serum Amyloid A1

Human serum amyloid A protein-1 (SAA-1) is a multifunctional apolipoprotein produced by hepatocytes in response to proinflammatory cytokines.  It is secreted as a 12 kDa, 104 amino acid (aa), nonglycosylated polypeptide that displaces apoA1 in the HDL₃ complex (SwissProt #P02735).  The SAA-1 gene is one of three SAA genes in human, and it shows multiple alleles that are race dependent.  The SAA-1 gene product differs from the SAA-2 gene product by only seven amino acids.  Circulating SAA-1 shows multiple proteolytically-generated isoforms, with anywhere from one-to-three amino acids being cleaved from either the N- or C-terminus.  Mature human SAA-1 is 72%, 82% and 72% aa identical to mature mouse, rabbit and hamster SAA-1, respectively.   

• Entrez Gene IDs:

  6288 (Human); 20208 (Mouse)

• Alternate Names:

  MGC111216; PIG4; SAA; SAA1; SAA2; serum amyloid A protein; Serum Amyloid A1; TP53I4; tumor protein p53 inducible protein 4